动脉粥样硬化相关LncRNA的研究
The Research on Long Noncoding RNA Related to Atherosclerosis
摘要: 目的：筛选并验证新的调控动脉粥样硬化的差异lncRNA，为该类疾病的治疗和预后提供新的思路。方法：1) 100 ug/ml ox-LDL刺激人主动脉血管平滑肌细胞24 h作为实验组，正常人主动脉血管平滑肌细胞作为对照组，质检合格后，Trizol法提取总RNA，进行高通量测序，筛选符合标准的表达上调的差异lncRNA。qPCR验证差异lncRNA。2) shRNA慢病毒载体感染HAVSMC，检测目的基因敲减后表达量。3) 将人主动脉血管平滑肌细胞分为正常组、ox-LDL处理组、ox-LDL + shBOLA3-AS1、ox-LDL + shPOC1B-AS1、ox-LDL + shGRXCR1-4，采用CCK8和Transwell法检测各组HAVSMC的增殖迁移能力，ELISA法测定胆固醇水平。结果：1) 初步筛选三种显著表达上调的lncRNA：BOLA3-AS1、POC1B-AS1、GRXCR1-4。2) 各敲减组中目标lncRNA的表达水平均显著降低(P < 0.05)。3) 下调三种差异基因表达后，与ox-LDL处理组相比，其增殖活性，迁移能力及胆固醇水平均显著降低(P < 0.01)。结论：LncRNABOLA3-AS1、POC1B-AS1、GRXCR1-4显著表达上调并参与调控动脉粥样硬化的进程。
Abstract: Objective: Filter and verify new regulatory atherosclerosis LncRNA to provide new ideas for the treatment and prognosis of such diseases. Methods: 1) 100 ug/ml ox-LDL stimulates human aortic blood vessel smooth muscle cells for 24 h as the experimental group, and normal people’s aortic blood vessel smooth muscle cells are used as a control group. After qualified quality inspection, to-tal RNA was extracted by Trizol method for high-throughput sequencing to screen the differential lncRNA with upregulated expression that met the criteria. Differential lncRNA was verified by qPCR. 2) HAVSMC was infected with shRNA lentiviral vector, and the expression of target gene was de-tected after knockdown. 3) Select the most effective interference targets, divide human aortic blood vessel smooth muscle cells into normal group, ox-LDL treatment group, ox-LDL + shBOLA3-AS1, ox-LDL + shPOC1B-AS1, ox-LDL + shGRXCR1-4. The CCK8 and Transwell Method were used to detect the proliferation migration capabilities of each group of HAVSMC, and the ELISA method was used to determine cholesterol levels. Results: 1) Preliminary screening of three significant expression of LncRNA: BOLA3-AS1, POC1B-AS1, GRXCR1-4. 2) The expression level of the target LncRNA in each knocking group was significantly reduced (P < 0.05). 3) After the three different genes are lowered, compared with the ox-LDL processing group, its proliferation activity, migration capabilities and cholesterol levels are significantly reduced (P < 0.01). Conclusion: LncRNA BOLA3-AS1, POC1B-AS1, GRXCR1-4 significantly increased and participated in the process of regulating atherosclerosis.
文章引用：汪志伟, 丁洋, 万圣云. 动脉粥样硬化相关LncRNA的研究[J]. 临床医学进展, 2022, 12(11): 10374-10382. https://doi.org/10.12677/ACM.2022.12111495

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