缺血再灌注损伤药物治疗时机的选择

Time Choosing of Drug Treatment for Ische-mia-Reperfusion Injury

摘要: 目的：以硫氧还蛋白作为药物干预因素探索再灌注损伤药物治疗的最佳时机选择，通过动物实验探讨再灌注损伤药物前处理与后处理与疗效的关系。方法：使用雄性Sprague-Dawley大鼠建立缺血再灌注损伤模型，将28只Sprague-Dawley雄性大鼠随机分为A、B、C、D四组，A组缺血再灌注前药物注射组，于缺血再灌注前2小时腹腔注射硫氧还蛋白(20 μg/kg)，B组缺血再灌注后药物注射组，于缺血再灌注后2小时腹腔注射硫氧还蛋白(20 μg/kg)，C组为缺血再灌注前盐水注射组，于缺血再灌注前2小时注射2 ml盐水，D组为缺血再灌注后盐水注射组，于缺血再灌注后2小时注射2 ml盐水。于再灌注后48小时后每组随机选择5只大鼠处死，取皮肤行HE染色计数炎细胞。于再灌注后7天，对各组剩余大鼠进行拍照，并使用图像处理软件计算皮肤坏死率。结果：A组坏死率低于C组，B组坏死率低于D组，B组坏死率低于A组，HE染色切片炎细胞计数结果A组低于C组，B组低于D组B组低于A组。结论：再灌注后药物处理可以得到更优疗效。

Abstract: Objective: Thioredoxin was used as a drug intervention factor to explore the best timing of drug treatment for reperfusion injury, and the relationship between drug pre-treatment and post- treatment of reperfusion injury and efficacy was explored through animal experiments. Materials and methods: Twenty-eight male Sprague-Dawley rats were randomly divided into four groups: group A, B, C, and D. Group A received drug injection before ischemia-reperfusion. Thioredoxin (20 μg/kg) was intraperitoneally injected 2 hours before ischemia-reperfusion. In group B, thioredoxin (20 μg/kg) was intraperitoneally injected 2 hours after ischemia-reperfusion. In group C, 2 ml sa-line was injected 2 hours before ischemia-reperfusion. In group D, 2 ml saline was injected 2 hours after ischemia-reperfusion. At 48 hours after reperfusion, 5 rats in each group were sacrificed, and the skin was taken for HE staining and the inflammatory cells were counted. At 7 days after reper-fusion, 2 rats in each group were photographed, and the percentage of skin necrosis was calculated. Result: The necrosis rate of group A was lower than that of group C, group B was lower than that of group D, and group B was lower than that of group A. The results of inflammatory cell count in HE stained sections of group A were lower than that of group C, group B was lower than that of group D, and group B was lower than that of group A. Conclusion: Drug treatment after reperfusion can get better results.