苦参对AS小鼠肠道菌群结构的影响

Effect of Sophora flavescens on Intestinal Flora Structure of AS Mice

摘要: 目的：通过16S rRNA高通量测序技术研究苦参对AS小鼠肠道菌群结构的影响。方法：ApoE-/-小鼠喂养高脂饲料10周建造动脉粥样硬化模型，同时分别设ApoE-/-小鼠和C57小鼠给予普通饲料喂养作为对照组，另设ApoE-/-小鼠预先给予苦参水溶液灌胃作为预处理组。10周后将造模小鼠随机分为模型组、阳性对照组及苦参高、中、低剂量组，与其余三组一起分别进行给药。给药结束后进行样本采集及肠道菌群测序。结果：各组小鼠肠道菌群结构存在显著差异。与对照组相比，模型组小鼠Lawsonibacter、肠鼠杆菌(Muribaculum)丰度降低，邓氏菌属(Duncaniella)丰度升高；与模型组相比，阳性对照组普雷沃氏菌属(Prevotella)、肠鼠杆菌(Muribaculum)丰度降低；与模型组相比，苦参给药组小鼠Lawsonibacter、泪滴形孢子属(Lacrimispora)、回肠杆菌属(Ileibacterium)丰度升高，邓氏菌属(Duncaniella)、普雷沃氏菌属(Prevotella)丰度降低。模型组小鼠氯代环己烷和氯苯降解途径(Chlorocyclohexane and chlorobenzene degradation)显著上升，苦参给药后丙酮酸代谢途径(Pyruvate metabolism)、柠檬烯和蒎烯降解途径(Limonene and pinene degradation)以及糖酵解/糖异生途径(Glycolysis/Gluconeogenesis)下降，果糖和甘露糖代谢(Fructose and mannose metabolism)通路显著上升。结论：苦参改变了动脉粥样硬化小鼠肠道菌群结构，主要的菌属有Prevotella、Lacrimispora等，并且苦参可能作用的通路有糖酵解/糖异生代谢途径及果糖和甘露糖代谢途径等。

Abstract: Objective: The effect of Sophora flavescens on intestinal microflora of AS mice was studied by 16S rRNA high-throughput sequencing technique. Method: ApoE-/- mice were fed with high-fat diet for 10 weeks to establish atherosclerotic model. At the same time, ApoE-/- mice and C57 mice were fed with normal diet as control group, and ApoE-/- mice were given Sophora flavescens solution as pre-treatment group. ApoE-/- mice were fed with high-fat diet for 10 weeks to establish atherosclerotic model. At the same time, ApoE-/- mice and C57 mice were fed with normal diet as control group, and ApoE-/- mice were given Sophora flavescens solution as pretreatment group. After administration, samples were collected and intestinal floras were sequenced. Results: There were significant dif-ferences in the structure of intestinal flora in each group. Compared with the control group, the abundance of Lawsonibacter and Muribaculum decreased and the abundance of Duncaniella in-creased in the model group. Compared with the model group, the abundance of Prevotella and Mu-ribaculum in the positive control group decreased. Compared with the model group, the abundance of Lawsonibacter, Lacrimispora and Ileibacterium in Sophora flavescens group increased, while that of Duncaniella and Prevotella decreased. Chlorocyclohexane and chlorobenzene degradation path-way increased significantly in model group. After administration of Sophora flavescens, pyruvate metabolism pathway, limonene and pinene degradation pathway and glycolysis/gluconeogenesis pathway decreased, while fructose and mannose metabolism pathway increased significantly. Con-clusion: Sophora flavescens can change the structure of intestinal flora in atherosclerotic mice, and the main bacteria are Prevotella, Lacrimispora and so on. The possible pathways of Sophora fla-vescens are glycolysis/gluconeogenesis and fructose and mannose metabolism.