基于网络药理学探究苦杏仁的治疗潜能及作用机制

Exploring the Therapeutic Potential and Mechanism of Semen Armeniacae Amarum Based on Network Pharmacology

摘要: 目的：基于网络药理学探究苦杏仁治疗疾病的潜能及作用机制。方法：从TCMSP中检索出苦杏仁的有效活性成分，收集其靶点；利用String数据库构建蛋白互作网络；使用Cytoscape软件进行可视化并分析筛选核心靶点；应用DAVID数据库进行GO功能及KEGG通路富集分析；应用CTD数据库预测苦杏仁治疗疾病的潜能；最后使用Cytoscape软件构建“药物–成分–靶点–通路–疾病”网络图。结果：最终获得76个苦杏仁活性成分，如苦杏仁苷、熊果酸、异甘草素、醇溶蛋白、薄荷醇、香叶醇等，作用靶点236个，涉及通路主要有：癌症、脂质和动脉粥样硬化、乙型肝炎、糖尿病并发症AGE-RAGE等，具有治疗肝硬化、乳腺肿瘤、糖尿病、白血病、淋巴瘤、前列腺肿瘤、关节炎等的潜能。结论：通过网络药理学的方法分析了苦杏仁的药效物质基础，挖掘了苦杏仁治疗肿瘤、糖尿病、白血病等疾病的潜能，为进一步研究奠定了基础。

Abstract: Objective: To explore the therapeutic potential and mechanism of Semen Armeniacae Amarumon multiple diseases based on network pharmacology. Methods: The active ingredient of Semen Ar-meniacae Amarum was retrieved from TCMSP and their targets were collected; Construction of the protein interaction network using the String database; Visualization and analytical screening of core targets using Cytoscape software; Application of CTD database to predict the therapeutic potential of Semen Armeniacae Amarum for diseases; Finally, Cytoscape software was used to construct a “drug-component-target-pathway-disease” network diagram. Results: 76 active ingredients of Semen Armeniacae Amarum were obtained, including Amygdalin, ursolic acid, isoliquiritigenin, Glabridin, menthol, geraniol and so on, corresponding to 236 targets. The main pathways involved are: cancer, Lipid and atherosclerosis, Hepatitis B, AGE-RAGE signaling pathway in diabetic complications and other signal pathways. It has the potential to treat liver cirrhosis, breast neoplasms, diabetes mellitus, leukemia, lymphoma, prostatic neoplasms, arthritis and other diseases. Conclusion: The material basis of the medicinal effects of Semen Armeniacae Amarum was analyzed through the method of network pharmacology, and the potential of Semen Armeniacae Amarum for the treatment of tumors, diabetes mellitus, leukemia and other diseases was explored, laying the foundation for its further research.