红景天苷通过抑制炎症因子减轻急性肺损伤

Salidroside Alleviates Acute Lung Injury by Inhibiting Inflammatory Factors

摘要: 目的：探讨红景天苷(Sal)对脂多糖(LPS)诱导的急性肺损伤(ALI)炎症因子的作用。方法：将36只SD大鼠随机分成6组，分别为生理盐水对照组(NS组)、5 mg/kg LPS损伤组(LPS组)、不同浓度的Sal处理组(10 mg/mL、40 mg/mL、80 mg/mL)、阳性对照组(5 mg/kg地塞米松组(DEX组))，每组6只。大鼠腹腔注射LPS构建急性肺损伤模型。造模6 h后观察大鼠一般活动状况，然后处死大鼠；取右下肺叶计算肺湿重和干重质量比值变化(W/D)；右肺上叶做组织匀浆，其余肺组织放在−80度冰箱冻存备用；BCA法测定肺组织中的总蛋白含量；酶联免疫吸附法(ELISA)测定TNF-α、IL-6、GM-CSF、ACE2的含量。结果：Sal处理后的大鼠一般活动状况明显改善；Sal处理后的大鼠肺W/D比值相比LPS组明显降低(P < 0.05)；Sal处理后的大鼠总蛋白含量的水平相比LPS组明显降低(P < 0.05)；Sal处理后的大鼠相比LPS组肺组织炎症因子(TNF-α、IL-6、GM-CSF)含量明显降低(P < 0.01)；Sal处理后的大鼠相比LPS组肺组织中ACE2的含量升高(P < 0.01)。结论：Sal通过抑制炎症因子的产生和提高ACE2的含量而对ALI具有治疗作用。

Abstract: Objective: To investigate the effect of salidroside (Sal) on inflammatory factors in lipopolysaccharide (LPS)-induced acute lung injury (ALI). Methods: Thirty-six SD rats were randomly divided into 6 groups: normal saline control group (NS group), 5 mg/kg LPS injury group (LPS group), different concentrations of Sal treatment groups (10 mg/mL, 40 mg/mL, 80 mg/mL), positive control group (5 mg/kg dexamethasone group (DEX group)), 6 rats in each group. Rat model of acute lung injury was established by intraperitoneal injection of LPS. After 6 hours of modeling, the general activity of rats was observed, and then the rats were sacrificed. The right lower lobe was taken to calculate the ratio of wet weight to dry weight (W/D). The upper lobe of the right lung was homogenized, and the rest of the lung tissue was stored in the refrigerator at −80˚C. BCA method was used to determine the total protein content in lung tissue. The contents of TNF-α, IL-6, GM-CSF and ACE2 were determined by enzyme-linked immunosorbent assay (ELISA). Results: After Sal treatment, the general activity of rats was significantly improved. The lung W/D ratio of Sal-treated rats was significantly lower than that of LPS group (P < 0.05). The total protein content of Sal-treated rats was significantly lower than that of LPS group (P < 0.05). The contents of inflammatory factors (TNF-α, IL-6, GM-CSF) in lung tissue of Sal-treated rats were significantly lower than those of LPS group (P < 0.01). ACE2 content in lung tissue of Sal-treated rats was increased compared with LPS group (P < 0.01). Conclusion: Sal has a therapeutic effect on ALI by inhibiting the production of inflammatory factors and increasing the content of ACE2.