一测多评法同时测定三七血伤宁散中5种皂苷类成分的含量

Simultaneous Determination of Five Saponins in Sanqi Xueshangning Powder by Quantitative analysis of Multi-Components with Single Marker Method

摘要: 目的：建立一测多评法(Quantitative analysis of multi-components by single-marker, QAMS)同时测定三七血伤宁散中人参皂苷Rg₁、人参皂苷Rb₁、人参皂苷Rd、重楼皂苷VII、重楼皂苷I，5种成分的含量。方法：采用Agilent5Tc-C18柱(250 mm × 4.6 mm, 5 μm)；流动相乙腈–水，梯度洗脱；体积流量1.1 mL/min；柱温25℃；检测波长210 nm，以人参皂苷Rb₁为内参物，建立其他四种成分的相对校正因子；通过一测多评法和外标法结果比较，验证一测多评法的准确性与实用性。结果：在一定线性范围内以人参皂苷Rb1为内参物，建立人参皂苷Rg₁、人参皂苷Rd、重楼皂苷VII、重楼皂苷I的相对校正因子分别为0.7210、0.9011、0.9023、0.7598。一测多评法与外标法含量测定结果无显著性差异。结论：一测多评法可用于三七血伤宁散中五种不同成分的含量同时测定。

Abstract: Objective: To establish a quantitative analysis of multi-components by single marker (QAMS) method for the simultaneous determination of five components (Ginsenoside Rg₁, Ginsenoside Rb1, Ginsenoside Rd, Polyphyllin VII, Polyphyllin I) in Sanqi Xueshangning powder. Methods: The analysis was performed on an Agilent5Tc-C18 column ( 250 mm × 4.6 mm, 5 μm) at 25˚C, with mobile phase composed of acetonitrile-water at a flow rate of 1.1 mL/min in gradient elution mode, and the detection wavelength was set at 210 nm. Using Ginsenoside Rb₁ as internal substance, the relative correlation factors (RCFs) of four Saponins were calculated. By comparing the results of the external standard method (ESM) and QAMS methods, the feasibility and accuracy of QAMS method were verified. Results: The relative correlation factors of ginsenoside Rg₁, ginsenoside Rd, Polyphyllin VII and Polyphyllin I to ginsenoside Rb₁ were 0.7210, 0.9011, 0.9023, 0.7598, respectively. There was no significant difference between the QAMS and ESM in 20 batches of samples. Conclusion: The QAMS method is accurate and easy to operate, and can be used for the content simultaneous determination of Sanqi Xueshangning Powder.