乙醇喂养小鼠诱导肝损伤模型的制作

Establishment of Liver Injury Model in Mice Fed with Ethanol

摘要: 目的：通过慢性酒精喂养加急性酒精灌胃来制作肝损伤的小鼠模型。方法：将雄性C57BL/6小鼠随机分为对照组和模型组。前十天每天给予对照组不含酒精液体饲料喂养，给予模型组酒精液体饲料喂养，第十一天给对照组灌胃麦芽糊精溶液(20 μL/Kg)，模型组灌胃酒精(20 μL/Kg)。实验结束后检测血清肝功能指标、脂质代谢相关指标，并观察肝脏组织病理变化。结果：与对照组小鼠比较，模型组小鼠血清中胆固醇(P < 0.0001，n = 14~15)、甘油三酯(P < 0.01, n = 14~15)和谷丙转氨酶(P < 0.01, n = 14~15)均显著升高；肝脏匀浆检测胆固醇和甘油三酯(P > 0.05, n = 14~15)在实验组中均高于对照组，且胆固醇(P < 0.01, n = 14~15)在两组间有显著差异。苏木精–伊红结果显示，与对照组相比，造模组的肝组织破坏程度较重。结论：模型组小鼠出现了肝细胞损伤，脂肪累积，运用慢性喂养加上急性灌胃的方法成功模拟酒精肝损伤，这对于之后的临床治疗和新机制的研究具有实践意义。

Abstract: Objective: A mouse model of liver injury was created by chronic alcohol feeding and acute alcohol gavage. Method: Male C57BL/6 mice were randomly divided into control group and model group. For the first ten days, the control group was fed with control liquid feed, and the experimental group was fed with alcohol liquid feed. On the 11th day, the control group was fed with maltodextrin solution (20 μL/kg), and the model group was fed with alcohol (20 μL/kg). After the experiment, serum liver function indexes and lipid metabolism-related indexes were detected, and the patho-logical changes of liver tissue were observed. Result: Compared with the control group, the serum cholesterol (P < 0.0001, n = 14~15), triglyceride (P < 0.01, n = 14~15) and alanine aminotransfer-ase (P < 0.01, n = 14~15) of the model group were significantly higher than those of the control group. Significantly increased; cholesterol and triglyceride (P > 0.05, n = 14~15) detected in liver homogenate were higher in the experimental group than in the control group, and there was a sig-nificant difference in cholesterol (P < 0.01, n = 14~15) between the two groups. The results of he-matoxylin-eosin showed that compared with the control group, the damage degree of liver tissue in the model group was more serious. Conclusion: The mice in the model group had liver cell damage and fat accumulation. The method of chronic feeding and acute gavage was used to successfully simulate alcoholic liver injury, which has practical significance for the subsequent clinical treat-ment and the study of new mechanisms.