高效液相色谱法测定化妆品中三肽-1铜的含量

Determination of Copper Tripeptide-1 in Cosmetics by High Performance Liquid Chromatography

摘要: 建立了高效液相色谱法测定化妆品中三肽-1铜含量的方法。分别对样品的前处理和色谱条件进行了优化，发现水剂类化妆品无需加入破乳剂，乳化类化妆品中需加入破乳剂才能将目标物质提取完全。方法学考察结果表明，三肽-1铜含量在5~500 mg/L范围内线性良好，相关系数可达0.9999，其回收率为92.5%~99.6%之间，相对标准偏差(RSD)在2.27%~3.25%之间，检出限为10 mg/kg。实验结果证明，该方法适于化妆品中三肽-1铜含量的测定。

Abstract: A method for the determination of copper tripeptide-1 in cosmetics by HPLC was established. The pretreatment of samples and chromatographic conditions were optimized respectively. It was found that demulsifiers were not necessary for aqueous cosmetics, and demulsifiers were needed for emulsified cosmetics to extract the target substances completely. The results of methodology investigation showed that the copper content of tripeptide-1 was linear within the range of 5-500 mg/L, the correlation coefficient was 0.9999, the recovery was 92.5%~99.6%, the relative standard deviation (RSD) was 2.27%~3.25%, and the detection limit was 10 mg/kg. The experimental results show that the method is suitable for the determination of copper tripeptide-1 in cosmetics.