青岛市健康新生儿血清钙、磷、碱性磷酸酶参考区间的研究

Study on the Reference Interval of Serum Calcium, Phosphorus and Alkaline Phosphatase of Healthy Newborn in Qingdao

摘要: 目的：建立青岛市健康新生儿血清钙、磷、碱性磷酸酶的参考值范围，从而为青岛市新生儿健康体检及疾病诊断提供依据。方法：收集2020年11月到2021年5月，在青岛市妇女儿童医院产科及新生儿科招募出生后1~28天新生儿作为参考人群，共计1614例纳入研究组，其中男926例，女688例。将其分为4个日龄组，分别为1~7天(864名)、8~14天(452名)、15~21天(173名)、22~28天(125名)。使用西门子Siemens advia 2400全自动生化检测仪检测所有新生儿的血清钙、磷、碱性磷酸酶浓度。比较和分析不同日龄组以及组中不同性别新生儿的上述指标水平，建立青岛地区不同日龄段健康新生儿参考值范围。结果：不同日龄组新生儿Ca、P、ALP水平比较差异均有统计学意义；各日龄组内不同性别新生儿的Ca、P、ALP水平比较差异均无统计学意义。确定各日龄组健康新生儿Ca、P、ALP的参考区间分别为：1~7天：Ca 1.98~2.61 mmol/L，P 1.51~2.84 mmol/L，ALP 88~279 U/L；8~14天：Ca 2.24~2.79 mmol/L，P 1.59~2.81 mmol/L，ALP 103~343 U/L；15~21天：Ca 2.26~2.86 mmol/L，P 1.51~2.71 mmol/L, ALP 151~379 U/L；22~28天：Ca 2.19~2.77 mmol/L，P 1.46~2.61 mmol/L，ALP 159~390 U/L。结论：初步建立了青岛市健康新生儿血清Ca、P和ALP的参考区间，对临床医生特别是新儿科医生对上述指标的结果解释和临床干预具有很好的参考价值。

Abstract: Objective: To establish the reference value range of serum calcium, phosphorus and alkaline phos-phatase of healthy neonates in Qingdao, so as to provide basis for health examination and disease diagnosis of neonates in Qingdao. Methods: From November 2020 to May 2021, 1614 neonates (926 males and 688 females) who were recruited from Obstetrics and Neonatal Department of Qingdao Women’s and Children’s Hospital from 0~28 days after birth were included in the study group. They were divided into four age groups: 1~7 days (864), 8~14 days (452), 15~21 days (173), and 22~28 days (125). The serum calcium, phosphorus, and alkaline phosphatase of all neonates were meas-ured with Siemens advia 2400 automatic biochemical detector to compare and analyze the above indicator levels of newborns of different age groups and genders in groups, and establish the nor-mal reference value range of newborns of different age groups in Qingdao. Results: The levels of Ca, P and ALP in neonates of different age groups were statistically significant; there was no significant difference in the levels of Ca, P and ALP among different sex newborns in each day age group. The normal reference values of Ca, P and ALP of neonates in each age group were determined as follows: 1~7 days: Ca 1.98~2.61 mmol/L, P 1.51~2.84 mmol/L, ALP 88~279 U/L; 8~14 days: Ca 2.24~2.79 mmol/L, P 1.59~2.81 mmol/L, ALP 103~343 U/L; 15~21 days: Ca 2.26~2.86 mmol/L, P 1.51~2.71 mmol/L, ALP 151~379 U/L; 22~28 days: Ca 2.19~2.77 mmol/L, P 1.46~2.61 mmol/L, ALP 159~390 U/L. Conclusion: The reference interval of serum calcium, phosphorus and alkaline phosphatase of healthy neonates in Qingdao has been preliminarily established, which has a good reference value for clinicians, especially new pediatricians, to interpret the results of the above indicators and to intervene in clinical practice.