分离胶真空采血管对血清学筛查指标测定的影响

Effect of Vacuum Blood Collection Tube with Separating Gel on Determination of Serological Screening Indexes

摘要: 目的：分析分离胶真空管采血管对唐氏血清学筛查指标测定的影响。方法：在全自动时间分辨荧光免疫仪对分离胶分离的早、中孕期母血清学产前筛查指标进行测定，并对测定结果与常规分离的血清管血清进行比较。结果：早孕期血清学筛查指标PAPP-A和HCG在两种分离方法测定结果之间比较，差异无统计学意义(P > 0.05)。中孕期血清学筛查指标AFP和uE3在两种分离方法测定结果之间比较，差异有统计学意义(P < 0.05)；中孕期血清学筛查指标β-hcg在两种分离方法测定结果之间比较，差异无统计学意义(P > 0.05)。结论：分离胶真空采血管影响AFP和uE3的测定结果，不适用于中孕期血清学筛查指标的测定，但可以用于早孕期血清学筛查指标的测定。

Abstract: Objective: To analyze the influence of vacuum tube blood collection tube with separating glue on the determination of down’s serological screening index. Methods: The maternal serological pre- natal screening indicators in the early and middle trimester of pregnancy separated by the separation gel were determined by automatic time-resolved fluoroimmunoassay, and the determined results were compared with conventionally separated serum samples. Results: There was no significant differ-ence between the results of the two separation methods in the determination of PAPP-A and HCG, two serological screening indicators during first trimester (P > 0.05). The differences of serum screening indexes AFP and uE3 during the second trimester between the two separation methods were statistically significant (P < 0.05). There was no significant difference in the determination re-sults of β-hcg, a serological screening indicator during the second trimester of pregnancy, between the two separation methods (P > 0.05). Conclusion: The vacuum blood collection tube of separation gel affected the determination results of AFP and uE3, and was not suitable for the determination of serological screening indicators during the second trimester, but it could be used for the determi-nation of serological screening indicators during the first trimester.