哈本即雾高透水光仪对传明酸体外透皮吸收的影响

Effect of Haben Aqua Atomization Skincare Pro on Transdermal Absorption of Tranexamic Acid in Vitro

摘要: 目的：观察哈本即雾高透水光仪的体外透皮能力，对传明酸体外透皮吸收的影响。方法：基于乳猪皮-Franz cell扩散池体系，采用哈本即雾高透水光仪不同档位针对乳猪背部皮肤进行Franz扩散池渗透性实验，采用液质联用方法对传明酸进行定量分析，测定不同时间点(6小时、24小时)扩散池中传明酸的累积渗透量Q、扩散百分率P、渗透系数Kp，考察样品对离体的小猪背部皮肤的透皮能力。结果：经透皮吸收测试，对照组涂抹组6 h、24 h时传明酸的累积渗透量分别为(0.8 ± 0.2) mg、(1.8 ± 0.2) mg，渗透率分别为3.9%、9.0%；哈本即雾高透水光仪–低档6 h、24 h传明酸的累积渗透量分别为(1.1 ± 0.7) mg、(2.3 ± 0.7) mg，渗透率为5.5%、11.5%，是对照组渗透率的1.4倍和1.3倍；中档6 h、24 h传明酸的传明酸的累积渗透量分别为(1.3 ± 0.2) mg、(2.6 ± 0.5) mg，渗透率为6.8%、13.0%，是对照组渗透率的1.7倍和1.4倍；高档6 h、24 h传明酸的传明酸的累积渗透量分别为(2.3 ± 1.2) mg、(4.3 ± 1.5) mg，渗透率为11.4%、21.4%，是对照组渗透率的2.9倍和2.4倍。与涂抹组相比，哈本即雾高透水光仪–高档24 h传明酸累积渗透量及渗透率均有显著提高(p < 0.05)。结论：哈本即雾高透水光仪可以促进传明酸的吸收，其中以高档位的作用最强。

Abstract: Objective: To observe the transdermal ability of Haben aqua Atomization skincare Pro in vitro and its effect on transdermal absorption of tranexamic acid in vitro. Methods: Based on the piglet skin Franz cell diffusion cell system, the Franz diffusion cell permeability experiment was carried out on the back skin of piglets at different positions with the Haben aqua Atomization skincare Pro. The quantitative analysis of tranexamic acid was carried out by LC-MS, and the cumulative permeability Q, diffusion percentage P, and permeability coefficient Kp of tranexamic acid in the diffusion cell at different time points (6 hours and 24 hours) were measured. The skin permeability of the sample to the back skin of piglets in vitro was investigated. Results: The transdermal absorption test showed that the accumulative permeation quantity of tranexamic acid is (0.8 ± 0.2) mg, (1.8 ± 0.2) mg, and the permeability was 3.9% at 6 h and 9.0% at 24 h in the control group respectively; The accumu-lative permeation quantity of tranexamic acid is (1.1 ± 0.7) mg, (2.3 ± 0.7) mg in Haben aqua Atom-ization skincare Pro of low grade 6 h and 24 h, and the permeability is 5.5% and 11.5% respective-ly, 1.4 times and 1.3 times higher than that of the control group; The accumulative permeation quantity of tranexamic acid is (1.3 ± 0.2) mg, (2.6 ± 0.5) mg in medium grade group 6 h and 24 h, and the permeability was 6.8% and 13.0% respectively, 1.7 times and 1.4 times of that of the con-trol group; The accumulative permeation quantity of tranexamic acid is (2.3 ± 1.2) mg, (4.3 ± 1.5) mg in high-grade group 6 h and 24 h, and the permeability was 11.4% and 21.4% respectively, 2.9 times and 2.4 times higher than that of the control group. Compared with the control group, the ac-cumulative permeation quantity and permeability of Haben aqua Atomization skincare Pro high-grade 24 h group tranexamic acid were significantly increased (p < 0.05). Conclusion: Haben aqua Atomization skincare Pro can promote the absorption of tranexamic acid, among which the ef-fect of high grade is the strongest.