CISD2基因对膀胱癌预后和诊断的系统性分析

Systematic Analysis of CISD2 Gene on the Prognosis and Diagnosis of Bladder Cancer

摘要: 目的：研究CISD2在膀胱癌(bladder cancer, BLCA)中的表达水平与生存预后的关系，预测患者的生存期，并分析CISD2的表达水平与免疫细胞浸润的相关性，从而研究该基因对肿瘤进展的影响。方法：用R分析CISD2在TCGA-BLCA数据库中的mRNA表达水平和临床数据，并对CISD2在膀胱癌中的共表达基因进行功能和信号通路富集分析。进一步用R语言分析CISD2与免疫细胞浸润的关系。结果：生物信息学分析表明CISD2在膀胱癌组织中表达水平显著高于相对应的癌周正常膀胱组织(P < 0.001)。CISD2的表达与Th2 cells、Th1 cells、aDC、NK CD56^dm cells、T helper cells、Tgd、Neutrophils、Macrophages等免疫细胞浸润呈正相关关系，与pDC、NK CD56^bright cells、Mast cells及B cells的浸润水平呈负相关关系。结论：CISD2在BLCA中可能与不良预后相关，并可能为BLCA的诊断和治疗提供新分子靶点。

Abstract: Objective: To investigate the relationship between CISD2 expression level and survival prognosis, and predict the survival time of patients in bladder cancer (BLCA), we analyzed the correlation be-tween CISD2 expression level and immune cell infiltration. Consequently, the effect of CISD2 on the development of tumor was studied. Methods: The mRNA expression profile and clinical data of CISD2 were obtained from TCGA-BLCA database by using R language. The function and signal path-way enrichment analysis of CISD2 and co-expressed genes in bladder cancer were further analyzed by using R language. The relationship between CISD2 expression and immune cell infiltration was further analyzed by using R language. Results: Compared with the normal group, CISD2 expression in BLCA was significantly increased (P < 0.001). High CISD2 expression predicted poor prognosis in BLCA. CISD2 expression level has significant positive correlations with infiltrating levels of Th2 cells, Th1 cells, aDC, NK CD56dm cells, T helper cells, Tgd, Neutrophils and Macrophages cells. In addition, CISD2 expression level has significant negative correlation with pDC, NK CD56 bright cells, Mast cells and B cells. Conclusion: This study implied that CISD2 may be a promising prognosis predictor and potential therapy target for BLAC patients.