夏枯草治疗甲状腺结节的作用机制潜在药理机制

Potential Pharmacological Mechanism of Action Mechanism of Spica prunellae in Treating Thyroid Nodule

摘要: 目的：确定夏枯草(Spica prunellae, SP)治疗甲状腺结节(thyroid nodule, TN)的有效性和潜在机制。方法：首先，从公开数据库搜集夏枯草活性成分对应靶点及甲状腺结节的靶点。通过网络拓扑分析、GO和KEGG通路富集分析筛选关键靶点和机制。应用分子对接来验证夏枯草与靶点的关系。结果：夏枯草试验组在治疗甲状腺结节可提高临床总有效率，经网络分析，6个靶点(AKT1、TP53、IL6、MAPK3、MAPK1、CASP3)被识别为关键治疗靶点。富集分析表明，潜在机制集中在与炎症、增殖和凋亡相关的生物过程和途径。关键途径被认为是PI3K-AKT信号通路。结论：本研究阐明SP可能通过PI3K-AKT途径抑制炎症和增殖并促进细胞凋亡，是一种潜在的治疗甲状腺结节的药物。

Abstract: Objective: This research is to identify the underlying and efficient mechanism of Spica prunellae (SP) in the treatment of thyroid nodule (TN). Methods: Firstly, the targets corresponding to the ac-tive components of Prunella vulgaris and the targets of thyroid nodules were collected from public databases. The key targets and mechanisms were screened by network topology analysis, GO and KEGG pathway enrichment analysis. Molecular docking was used to verify the relationship between Prunella vulgaris and target. Results: Meta-analysis revealed that Prunella vulgaris significantly improved the total clinical effective rate in the treatment of thyroid nodules, network pharmacology suggested that 6 targets (AKT1, TP53, IL6, MAPK3, MAPK1, CASP3) were identified as dominating therapeutic targets after network analysis. The results of enrichment analysis suggested that the potential mechanism was concentrated mainly on the biological processes and pathways associated with inflammation, proliferation, and apoptosis. Especially, the main pathway was regarded as the PI3K-AKT signaling pathway. Conclusion: SP may suppress tumor, inflammation and proliferation and promote apoptosis through the PI3K-AKT pathway, which makes SP a potential treatment against TN.