基于多重LRET效应的上转换发光探针构筑及pH检测
A Multiple LRET-Based Upconversion Luminescent Probe for Sensitive pH Detection
DOI: 10.12677/AAC.2023.131001, PDF,    国家自然科学基金支持
作者: 陈婷婷, 秦淑恒, 张玉明*：南通大学化学化工学院，江苏 南通
关键词: pH检测；上转换发光探针；LRET；pH Detection； Upconversion Luminescent Probe； LRET
摘要: pH异常与多种疾病密切相关，酸化是所有实体肿瘤的特征性标志之一。上转换发光材料具有多种独特优势，适合生命体物种成像。有机小分子CouDa负载到上转换纳米颗粒(UCNPs)表面的疏水层组装成UCNPs@CouDa探针，酸性/碱性条件下，能量受体CouDa与供体UCNPs之间产生多重发光共振能量转移(LRET)效应，且随pH变化的趋势相反。探针对溶液pH检测的灵敏度高，pKa约7.3，线性响应范围在6.0~8.5，不受生命体常见物种的干扰。与小分子探针CouDa相比，该纳米探针响应速率快，线性响应范围宽，表明疏水环境有利于这一亲核加成反应机理的pH检测。
Abstract: pH dysregulation is closely relevant to many diseases, and acidosis is a hallmark of all solid tumors. Upconversion luminescent particles (UCNPs) are suitable for bio-imaging due to their typical advantages. UCNPs@CouDa probe was constructed with CouDa in the hydrophobic layer on UCNPs surface. Multiple luminescence resonance energy transfer (LRET) processes occurred between CouDa and UCNPs that changed oppositely with pH variation. UCNPs@CouDa probe could image pH fluctuations with high sensitivity and good selectivity. Its pKa was nearly 7.3 and the linear detection range was between 6.0 to 8.5 which was broader than that of the small molecule probe CouDa. The nanoprobe responded to pH changes more quickly. All these indicated that the hydrophobic environment was favorable for the pH imaging, which is based on a nucleophilic reaction mechanism.
文章引用：陈婷婷, 秦淑恒, 张玉明. 基于多重LRET效应的上转换发光探针构筑及pH检测[J]. 分析化学进展, 2023, 13(1): 1-10. https://doi.org/10.12677/AAC.2023.131001

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