PNPLA3 rs3747207位点多态性与NAFLD易感性的相关性

Correlation between PNPLA3 rs3747207 Polymorphism and Non-Alcoholic Fatty Liver Disease

摘要: 目的：探讨青岛地区汉族人群中patatin样磷脂酶结构域蛋白3 (Patatin-like phospholipase domain- containin, PNPLA3)基因rs3747207位点多态性与非酒精性脂肪性肝病(Non-Alcoholic Fatty Liver Disease, NAFLD)发病风险的相关性。方法：随机纳入2020年12月~2022年6月就诊于青岛市市立医院的NAFLD患者223例，并选取同期健康对照人群168例。采集所有受试者的临床数据及血液样本，检测血液样本的生物化学指标并测定PNPLA3 rs3747207位点基因型。根据是否符合正态分布对定量资料进行t检验或非参数检验。定性资料两组间比较采用χ2检验。采用二元logistic回归分析NAFLD发生的危险因素。结果：PNPLA3 rs3747207基因型分布及等位基因频率在NAFLD组和对照组之间分布无统计学意义(P均 > 0.05)。二元logistic回归分析表明，携带GG基因型未增加NAFLD发病风险(OR = 0.702, 95%CI: 0.428~1.152, P = 0.161)。GG基因型携带者低密度脂蛋白水平高于非携带者(P < 0.05)。结论：PNPLA3 rs3747207多态性与青岛地区汉族人群NAFLD发病风险无明显相关性。

Abstract: Objective: To investigate the relationship between PNPLA3 rs3747207 polymorphism and the on-set risk of non-alcoholic fatty liver disease in the Chinese Han population in Qingdao. Methods: A to-tal of 223 patients with NAFLD who attended Qingdao Municipal Hospital from December 2020 to June 2022 were enrolled in NAFLD group; 168 healthy individuals were enrolled in control group. We collected the clinical date and blood sample of all subjects to measure related biochemical pa-rameters and detect PNPLA3 rs3747207 genotype. The t-test was used for the comparison of nor-mally distributed quantitative data between two groups, and the non-parametric test was used for the non-normally distributed quantitative data between two groups. The chi-square test was used for comparison of qualitative data between two groups. The binary logistic regression analysis was used to investigate the risk factors for NAFLD. Result: There was no significant difference in PNPLA3 rs3747207 genotype distribution and allele frequency between the control group and NAFLD group (P > 0.05). The binary logistic regression analysis showed that the risk of NAFLD in individuals with genotype GG was not increased compared with those in individuals with allele gene A (OR = 0.702, 95%CI: 0.428~1.152, P = 0.161). For all subjects, the subjects with GG genotype had a higher level of low density lipoprotein than those with allele gene A (P < 0.05). Conclusion: There is no significant association between PNPLA3 rs3747207 polymorphism and the risk of NAFLD in the Chinese Han population in Qingdao.