基于网络药理学探究淡豆豉对缺血性卒中的作用机制

To Investigate the Mechanism of Sojae Semen Praeparatum Sauce on Ischemic Stroke Based on Network Pharmacology

摘要: 目的：通过网络药理学探讨淡豆豉对缺血性卒中(Ischemic stroke, IS)的作用机制。方法：通过TCMSP数据库和Uniprot对淡豆豉的有效成分和靶点进行筛选；使用OMIM、DisGeNET、GeneCards数据库收集IS相关靶点。将IS相关靶点与淡豆豉有效成分靶点的交集作为淡豆豉防治IS的潜在作用靶点，借助STRING数据库和Cytoscape3.9.1软件的“CytoNCA”绘制PPI图，利用DAVID 2021数据库和微生信平台进行GO和KEGG可视化分析。结果：筛选出淡豆豉中含有5个活性成分及34个AS潜在作用靶点，其中蛋白互作显示ESR1、PPARG、GSK3B等是淡豆豉防治IS的关键靶点；KEGG显示淡豆豉主要通过IL-17信号通路来发挥防治AS的作用。结论：本研究初步探讨了淡豆豉治疗IS，可能是通过作用于ESR1、PPARG、GSK3B等关键靶点和IL-17信号通路以及其他疾病，进而发挥治疗缺血性卒中的作用。

Abstract: Objective: To investigate the mechanism of Sojae Semen Praeparatum on ischemic stroke (IS) by network pharmacology. Methods: The active ingredients and targets of Sojae Semen Praeparatum were screened by TCMSP database and Uniprot, and the IS-related targets were collected by OMIM, DisGeNET and GeneCards databases. The intersection of IS-related targets and active ingredient targets of Sojae Semen Praeparatum was used as a potential target for the prevention and treatment of IS by Sojae Semen Praeparatum. PPI maps were drawn with the help of STRING database and “CytoNCA” of Cytoscape3.9.1 software, and GO and KEGG visualization analyses were performed using DAVID 2021 database and Microbiology Information platform. Results: Sojae Semen Praeparatum contained 5 active components and 34 potential targets of AS, of which ESR1, PPARG and GSK3B were the key targets of Sojae Semen Praeparatum to prevent and treat IS, and KEGG showed that Sojae Semen Praeparatum mainly played a role in preventing and treating AS through IL-17 signaling pathway. Conclusion: This study preliminarily investigated the therapeutic effect of Sojae Semen Praeparatum on IS, which may play a role in the treatment of ischemic stroke by acting on key targets such as ESR1, PPARG, GSK3B, IL-17 signaling pathway and other diseases.