纤维素酶辅助提取烟叶黄酮最佳工艺研究

Study on the Optimal Process ofCellulase-Assisted Extraction of Flavonoids from Tobacco Leaves

摘要: 烟叶粉经纤维素酶预处理后，采用乙醇溶液加热回流提取烟叶中的黄酮，并采用UV-VES测定在510 nm波长下的黄酮吸收值。采用单因素实验，探讨了料液比、酶解温度、酶用量、酶解时间对烟叶黄酮的吸光值的影响；并对以上四因素分别进行L₉(3⁴)正交实验，以黄酮吸光值为指标确定适宜的正交试验参数，得到酶辅助提取烟叶中黄酮的最佳提取工艺。研究表明，纤维素酶辅助提取烟叶中黄酮最佳工艺为：料液比1:26，酶用量4%，酶解温度50℃，酶解时间2.5 h。

Abstract: Tobacco leaf powder pretreated with cellulase, the flavonoids in tobacco leaves are extracted by heating and refluxing with ethanol solution, the absorption value of flavonoids at 510 nm wavelength was measured by UV-VES. Using one-factor experiments, the effects of solid-liquid ratio, enzymatic hydrolysis temperature, enzyme dosage and enzymatic hydrolysis time on the absorbance of tobacco flavonoids were discussed; the L₉(3⁴) orthogonal experiment was performed on the above four factors, respectively, the appropriate orthogonal test parameters were determined with the flavonoid absorbance value as an indicator, the optimal extraction process of enzyme-assisted extraction of flavonoids from tobacco leaves was obtained. Research shows that the optimal process of cellulase-assisted extraction of flavonoids from tobacco leaves is as follows: The solid-liquid ratio was 1:26, the enzyme dosage was 4%, the enzymatic hydrolysis temperature was 50˚C, and the enzymatic hydrolysis time was 2.5 h.