细胞污染及预防

Cell Contamination and Prevention

摘要: 细胞的质量决定实验的进程与结果，细胞污染不仅仅是造成实验室的损失，更严重的后果是导致数据的错误、实验的失败。因此，我们不仅要知道细胞培养过程中常见的细胞污染类型，更要熟悉它们的特征及鉴定方法。同时，了解细胞污染的来源后才能更精准地进行细胞污染的预防。当稀少且价值高的细胞受到污染时还要掌握其清除方法。但被污染过的细胞本身还是会或多或少的受到影响，防患于未然，正确操作，定期检查清理消毒培养设施及严格执行无菌操作规范才是关键。

Abstract: The quality of the cell determines the process and results of the experiment. Cell pollution not only causes the loss of the laboratory, but also leads to the error of the data and the failure of the experiment. Therefore, we should not only know the common types of cell contamination in cell culture, but also be familiar with their characteristics and identification methods. At the same time, the prevention of cell pollution can be more accurate only after understanding the source of cell pollution. When rare and valuable cells become contaminated, it is important to know how to remove them. But the contaminated cells themselves will be more or less affected. We should take preventive measures, and correct operation, regular inspection of cleaning and disinfection culture facilities and strict implementation of aseptic operation specifications are the key.