PCPA失眠大鼠大脑皮质中PACAP信号的表达及酸枣仁汤的干预作用

Expression of PACAP Signals in the Cerebral Cortex of PCPA Insomnia Rats and the Interaction Effect of Suanzaoren Decoction

摘要: 目的：采用氯苯丙胺酸(PCPA)制作失眠大鼠模型，观察其大脑皮质中的垂体腺苷酸环化酶激活肽(PACAP)、PACAP R1A以及受体后信号蛋白PKC mRNA表达变化情况及酸枣仁汤(SZRD)的干预作用。方法：64只大鼠分成7天正常组，7天对照组、7天PCPA组、15天正常组、15天对照组、15天PCPA组、SZRD组、PCPA + SZRD组，腹腔注射PCPA，予酸枣仁汤灌胃治疗，应用RT-PCR测定PACAP、PACAPR1A以及PKC mRNA表达含量的变化。结果：7天PCPA组、15天PCPA组大鼠大脑皮质中PACAP、PACAPR1A及PKC mRNA的表达量均显著降低(P < 0.05)。SZRD干预治疗后，三者相对表达量则显著增加。结论：PCPA致失眠的机制可能与PACAP信号表达含量的降低有关，SZRD能够促使PACAP信号表达增加，从而达到治疗失眠的效果。

Abstract: Objective: The insomnia rat model was established by chloramphetamine acid (PCPA). The changes of PACAP, PACAP R1A and PKC mRNA expression in the cerebral cortex and the intervention effect of SZRD were observed. Methods: Sixty-four rats were divided into 7 days normal group, 7 days control group, 7 days PCPA group, 15 days normal group, 15 days control group, 15 days PCPA group, SZRD group and PCPA + SZRD group. RT-PCR was used to detect the mRNA expression of PACAP, PACAPR1A and PKC. Results: The expression of PACAP, PACAPR1A and PKC mRNA in the cerebral cortex of rats in the 7-day PCPA and 15-day PCPA groups were significantly reduced (P < 0.05). The relative expressions of PACAP, PACAPR1A and PKC mRNA were significantly increased after the intervention of SZRD. Conclusions: The mechanism of PCPA causing insomnia may be related to the decrease of PACAP signal expression, and SZRD can induce the increase of PACAP signal expression, thus achieving the effect of treating insomnia.