天然产物研究中的掺杂组分——以毛鼠刺为例

Doped Components in Study of Natural Products—A Case Study of Itea Indochinensis

摘要: 目的：以毛鼠刺为例，分析总结天然产物研究中掺杂组分产生的原因。方法：采用色谱技术进行化学成分研究，基于波谱数据分析化合物结构，通过文献分析总结掺杂组分产生的原因。结果：从毛鼠刺枝叶的乙酸乙酯部位中共分离鉴定5个掺杂组分，分别为吲哚美辛(1)、吲哚美辛甲酯(2)、吲哚美辛乙酯(3)、邻苯二甲酸二丁酯(4)、对苯二甲酸二(2-乙基)己酯(5)。结论：化合物1~5均为掺杂组分，推测化合物1~3主要为农残引入，4~5为主要为提取过程中引入。

Abstract: Objective: To analyze and summarize the reasons for the generation of doped components in the natural products study of Itea indochinensis. Methods: Various chromatographic methods were applied during separation process; the structures were elucidated based on extensive spectral analyses; the reasons for the production of doped components were proposed via literature analyses. Results: Five compounds were isolated and identified as indometacin (1), indometacin methyl ester (2), indometacin ethyl ester (3), dibutyl phthalate (4), terephthalic acid bis (2-ethyl-hexyl) ester (5) from the EtOAc fraction of I. indochinensis ethanol extract. Conclusion: Compounds 1~5 were determined as doped components from I. indochinensis. It was speculated that compounds 1~3 were introduced by pesticide residues and 4~5 were mainly introduced during the extraction process.