复元活血汤治疗III型前列腺炎的网络药理学研究

Network Pharmacology of Fuyuan Huoxue Tang in the Treatment of Type III Prostatitis

摘要: 目的：通过网络药理学方法探讨复元活血汤治疗III型前列腺炎的作用机制。方法：使用中药系统药理学分析平台(TCMSP)获取复元活血汤的活性成分及药物靶点，从GeneCards、DisGeNET、OMIM、Drugbank数据库中筛选III型前列腺炎的疾病靶点，并与复元活血汤的药物靶点相映射取交集。通过STRING数据库和Cytoscape3.9.1软件构建中药–活性成分–靶点网络及PPI网络，并分析网络中的活性成分与关键靶点。利用R语言相关程序包进行GO功能、KEGG通路富集分析。结果：复元活血汤含有183种活性成分，对应的药物靶点有242个。复元活血汤治疗III型前列腺炎的潜在靶点有123个；主要活性成分有槲皮素、木犀草素、山奈酚、柚皮素等；关键靶点有丝裂原活化蛋白激酶14 (MAPK14)、转录因子p65 (RELA)、信号转导和转录激活因子3 (STAT3)等。GO功能富集分析涉及1757个生物过程、154个细胞组和98个分子功能；KEGG通路富集分析涉及192条信号通路。结论：复元活血汤可通过多种中药成分和多个靶点影响肿瘤坏死因子(TNF)、核因子κ B (NF-κ B)、toll样受体、丝裂原活化蛋白激酶(MAPK)等信号通路，治疗III型前列腺炎。

Abstract: Objective: To investigate the mechanism of action of Fuyuan Huoxue Tang in the treatment of type III prostatitis through a network pharmacology approach. Methods: The active ingredients and drug targets of Fuyuan Huoxue Tang were obtained using the Traditional Chinese Medicine Systematic Pharmacology Analysis Platform (TCMSP), and the disease targets of type III prostatitis were screened from the GeneCards, DisGeNET, OMIM, and Drugbank databases and mapped to the drug targets of Fuyuan Huoxue Tang to obtain the intersection. Construct TCM-active ingredient-target network and PPI network by STRING database and Cytoscape 3.9.1 software, and analyze the active ingredients and key targets in the network. Use the R language-related program package for GO function, analysis by KEGG pathway enrichment. Results: Fuyuan Huoxue Tang has sub 183 active ingredients and 242 corresponding drug targets. There were 123 potential targets of Fuyuan Hu-oxue Tang for the treatment of type III prostatitis; the main active ingredients were quercetin, lig-nocaine, kaempferol, naringenin, etc.; the key targets were MAPK14, RELA, STAT3, etc. The GO function enrichment analysis involved 1757 biological processes, 154 cell groups and 98 molecular functions; the KEGG pathway enrichment analysis involved 192 signaling KEGG pathway enrich-ment analysis involved 192 signaling pathways. Conclusion: Fuyuan Huoxue Tang may be used to treat type III prostatitis by affecting TNF, NF-κ B, Toll-like receptors, MAPK and other signaling pathways through multiple herbal components and multiple targets.