高胡萝卜素含量的盐藻藻种筛选

Screening of Dunaliella salina with High Carotene Content

摘要: 表观遗传学指明，不同生物可能会根据环境的不同而引起可遗传的基因表达或细胞表型变化。本文主要进行了筛选在滨州无棣海盐田中发现的高β-胡萝卜素含量的杜氏盐藻藻种的相关工作。海盐田为饱和盐水，水样中主要含有杜氏盐藻、嗜盐细菌和少量原生动物。首先由于杜氏盐藻具有鞭毛，所以通过沉淀法去除嗜盐细菌，进而筛选出上层水样中的杜氏盐藻，并将其接种到适宜浓度的海水培养基中使其生长。在积累一定生物量之后，提高盐度使得细胞开始积累胡萝卜素。最终得到高胡萝卜素含量的杜氏盐藻藻种。

Abstract: Epigenetics shows that, depending on the environment, different organisms may cause heritable gene expression or cell phenotype changes. In this article, we mainly screened Dunaliella with high carotene content found in sea salt fields in Wudi Binzhou. The sea salt field is saturated brine, and the water sample mainly contains Dunaliella salina, halophilic bacteria and a small amount of protozoa. First, since Dunaliella able to have flagella movement, halophilic bacteria can be easily removed by precipitation. The Dunaliella salina in the upper water sample is screened out, and then it is inoculated into a seawater medium of appropriate concentration for growth. After accumulating a certain amount of biomass, the salinity is increased, so that the Dunaliella salina cells begin to accumulate carotene. Finally, the species of Dunaliella salina with high carotene content was screened out.