BGN基因在膀胱癌进展与转移中的作用机制研究

The Mechanism of BGN in the Progression and Metastasis of Bladder Cancer

摘要: 目的：探讨双糖链蛋白聚糖(BGN)基因在膀胱癌进展与转移中的作用机制及与免疫浸润的关系。方法：收集TCGA数据库关于BGN的数据信息并利用R语言及SPSS进行统计分析。运用TIMER 2.0数据库分析BGN与免疫细胞浸润的关系。结果：利用TCGA数据库进行分析显示，与癌旁组织相比，BGN在膀胱癌组织中呈高表达(P < 0.05)，且在人种、性别、病理学分期中等不同亚组表达中具有统计学差异(P < 0.05)。BGN的表达与巨噬细胞、NK细胞、B细胞、DC细胞、Treg细胞等多种免疫细胞呈正相关。富集分析显示其在膀胱中的表达可能与G蛋白偶联受体、中性粒细胞脱颗粒信号通路相关。结论：BGN在膀胱癌组织中高表达，且与膀胱癌预后显著相关，与免疫浸润相关，可能为膀胱癌基因治疗提供新的靶点及更新免疫学说理论。

Abstract: Objective: To investigate the mechanism of BGN gene in the progression and metastasis of bladder cancer and its relationship with immune invasion. Methods: The data information about BGN in TCGA database were collected and analyzed by R language and SPSS. TIMER 2.0 database was used to analyze the relationship between BGN and immune cell infiltration. Results: TCGA database analysis showed that BGN was highly expressed in bladder cancer tissues compared with adjacent tissues (P < 0.05), and there were statistical differences in different subgroups such as race, gender and pathological stage (P < 0.05). The expression of BGN was positively correlated with macrophag-es, NK cells, B cells, DC cells, Treg cells and other immune cells. Enrichment analysis showed that its expression in bladder may be closely related to G protein-coupled receptor and neutrophil degran-ulation signaling pathway. Conclusion: BGN is highly expressed in bladder cancer tissues, and is sig-nificantly associated with the prognosis of bladder cancer, and is associated with immune invasion. BGN may provide a new target for gene therapy of bladder cancer and update immune theory.