小球藻–螺旋聚孢霉共生体构建工艺研究
Study on the Construction Technology of the Symbiote of Chlorella vulgaris and Clonostachys rosea
DOI: 10.12677/HJCET.2023.133017, PDF,   
作者: 许逊锋：浙江工业大学药学院，浙江 杭州；邵立强, 李开艳：嘉兴南湖学院新材料工程学院，浙江 嘉兴
关键词: 微藻–真菌共生体；构建工艺；生物净化；Algal-Bacteria Symbiote； Construction Technology； Biological Purification
摘要: 微藻–真菌共生体在净化废水和生物固碳方面已成为一种具有潜力的新型生物处理技术。相比单一微藻而言，藻菌共生可实现微藻和真菌在代谢功能互补，强化废水中营养物质摄取和CO2固定以及利于收获和降低成本。本文以普通小球藻和螺旋聚孢霉为实验材料，以成球率、生物量和叶绿素a (Chl-a)含量为考察指标，筛选不同转速、藻菌接种比、光强和GR24 (一种独脚金内酯人工合成类似物)浓度等因素对小球藻–螺旋聚孢霉共生体系构建的影响。结果发现，在160 rpm转速下，藻菌接种比为1:30时，藻菌球成球率和均一性最佳；在6000 lux光强下，添加10−7 M GR24可有效提高小球藻光合性能和藻菌生物量。这为人工构建微藻–真菌共生体提供了一定的技术支撑。
Abstract: Algal-bacteria symbiote has become a potential biological treatment technology for wastewater purification and carbon sequestration. Compared with a single microalgae treatment technology, algal-bacteria symbiote can realize complementary metabolic functions between algae and fungi, enhance nutrient uptake and CO2 fixation in wastewater, and facilitate harvest and cost reduction. In this paper, Chlorella vulgaris and Clonostachys rosea were used as experimental materials, and the pellet formation rate, biomass and chlorophyll a (Chl-a) content were used as inves-tigation indexes to screen the effects of rotating speeds, algal/bacterial inoculation ratio, light intensity and GR24 (a synthetic analog of strigolactone) concentration on the construction of the symbiosis system. The results showed that when the inoculating ratio was 1:30 at 160 rpm, the pellet formation rate and uniformity of algal bacteria were the best. Under a light intensity of 6000 lux, adding 10−7 M GR24 can effectively improve the photosynthetic performance and biomass. The result can provide technical support for the artificial construction of algal-bacteria symbiote.
文章引用：许逊锋, 邵立强, 李开艳. 小球藻–螺旋聚孢霉共生体构建工艺研究[J]. 化学工程与技术, 2023, 13(3): 147-154. https://doi.org/10.12677/HJCET.2023.133017

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