应用荧光法快速检测土霉素含量

Rapid Detection of Oxytetracycline Content by Fluorescence Method

摘要: 抗生素是药剂学中使用最广泛的药物类型之一。然而，由于抗生素耐药性的威胁，这些药物的效率最近有所下降。导致抗生素耐药性的重要因素之一是动物过度使用抗菌剂。因此，检测动物源性食品中的抗生素至关重要。本文将四苯基-1,3-丁二烯制成荧光探针，探究抗生素对固体TPB的猝灭效果。结果表明，土霉素对TPB高度猝灭；体系荧光强度与土霉素浓度成反比，且在浓度高于0.005 mol∙L−1时，荧光猝灭效应明显；四氢呋喃水溶液和正己烷水溶液体系下，土霉素对TPB高度猝灭；酸性和中性条件下，土霉素猝灭效果较好。因此，可以将此技术应用于快速检测土霉素，从而建立了定量抗生素测定的巨大潜力。

Abstract: Antibiotics are one of the most widely used drugs in pharmaceutics. However, due to the threat of antibiotic resistance, the efficiency of these drugs has recently declined. One of the important factors leading to antibiotic resistance is the excessive use of antibiotics in animals. Therefore, the detection of antibiotics in animal-derived foods is very important. In this paper, tetraphenyl-1,3- butadiene was used as a fluorescent probe to investigate the quenching effect of antibiotics on solid TPB. The results showed that oxytetracycline highly quenched TPB; The fluorescence intensity of the system is inversely proportional to the concentration of oxytetracycline, and the fluorescence quenching effect is obvious when the concentration is higher than 0.005 mol∙L−1; In the system of tetrahydrofuran aqueous solution and n-hexane aqueous solution, oxytetracycline highly quenched TPB; Under acidic and neutral conditions, oxytetracycline has better quenching effect. Therefore, this technology can be applied to the rapid detection of oxytetracycline, thus establishing a huge potential for quantitative antibiotic determination.