富血小板血浆可减轻膝骨性关节炎神经病理性疼痛并下调ATF3表达

Platelet-Rich Plasma Can Alleviate Neuropathic Pain and Down-Regulate ATF3 Expression in Knee Osteoarthritis

摘要: 目的：本研究旨在观察富血小板血浆(PRP)对单碘乙酸钠(MIA)诱导的膝骨关节炎(KOA)神经病理性疼痛大鼠镇痛疗效，并对其镇痛机制进行初步探讨。方法：向大鼠左膝关节注射60 µL MIA (80 mg/mL)诱导关节退变，造模前1天及造模后第1、3、7、14天对大鼠机械痛撤足阈值、热痛撤足阈值进行测定，在造模后15、17、19天MIA + PRP组连续3天予以膝关节PRP注射治疗，MIA + NS组予以等量生理盐水注射治疗，造模后21、28天对大鼠机械痛撤足阈值、热痛撤足阈值再次进行测定，使用免疫组化染色测定背根神经节激活转录因子3 (ATF3)表达。结果：MIA造模后大鼠机械痛撤足阈值、热痛撤足阈值显著降低，予以PRP注射治疗后，MIA + PRP组机械痛撤足阈值、热痛撤足阈值显著高于MIA + NS组。造模后28天，MIA + PRP组背根神经节中ATF3蛋白表达较MIA + NS组显著降低。结论：PRP可以减轻膝骨性关节炎大鼠神经病理性痛，这一作用可能是通过减轻神经损伤实现的。

Abstract: Objective: To observe the analgesic effect of platelet-rich plasma (PRP) on neuropathic pain in rats with knee osteoarthritis (KOA) induced by monosodium iodoacetate (MIA), and to explore its anal-gesic mechanism. Methods: 60 µl MIA (80 mg/mL) was injected into the left knee joint of rats to in-duce joint degeneration. Mechanical and thermal pain thresholds were measured at 1 day before modeling and 1, 3, 7, and 14 days after modeling. At 15, 17 and 19 days after modeling, the knee joints in MIA + PRP group were injected with PRP, and the knee joints in MIA + NS group were in-jected with the same amount of normal saline. The paw withdrawal threshold (PWT) and paw with-drawal latency (PWL) were measured again 21 and 28 days after modeling, and the expression of activating transcription factor 3 (ATF3) in dorsal root ganglion (DRG) was detected by immuno-histochemical staining. Results: The mechanical pain threshold and thermal pain threshold of MIA rats were significantly decreased after modeling. After PRP injection, the PWT and PWL of MIA + PRP group were significantly higher than those of MIA + NS group. 28 days after modeling, the ex-pression of ATF3 in the dorsal root ganglia of the MIA + PRP group was significantly lower than that of the MIA + NS group. Conclusion: PRP can alleviate NP in rats with knee OA possibly by reducing nerve injury.