木质纤维素酶诱导物对平菇菌包发菌期的影响
Effects of Lignocellulase Inducer to Oyster Mushroom Spawning Management Period
DOI: 10.12677/HJAS.2023.135053, PDF,    科研立项经费支持
作者: 潘戈帆:湖南科技大学生命科学与健康学院,湖南 湘潭;许爱清*:湖南科技大学生命科学与健康学院,湖南 湘潭;重金属污染土壤生态修复与安全利用湖南省普通高等学校重点实验室,湖南 湘潭
关键词: 平菇发菌管理期木质纤维素酶微晶纤维素纤维二糖Oyster Mushroom Spawning Management Period Lignocellulase Microcrystalline Cellulose Cellobiose
摘要: 在当前转化利用农林废弃生物质资源工厂化生产平菇菌包过程中存在菌包发菌期长的实际问题,缩短菌包发菌期是菌包生产企业的现实需要。向平菇液体菌种培养体系添加适宜的木质纤维素酶系诱导物,包括微晶纤维素、纤维二糖、羧甲基纤维素钠或木质素磺酸钠,对比分析菌包发菌期时长和出菇产量指标。结果表明在I期液体菌种培养物能正常接种前24 h添加微晶纤维素(0.1%)或纤维二糖(0.1%)作为诱导剂,既都能缩短菌包发菌期3天,又能提升第一潮菇产量4%或11%。建立了缩短平菇菌包发菌期技术,能为菌包生产企业提质增效提供参考。
Abstract: There exists a problem of long spawning management period when factory production of the oyster mushroom spawning bags based on the agriculture and forestry waste biomass resources in the present, therefore the shortening of the period of spawning management is the reality demands for the spawning bags producing enterprise. The lignocellulase inducer such as microcrystalline cellu-lose, cellobiose, sodium carboxymethyl cellulose and sodium ligninsulfonate was added into the oyster mushroom liquid culture system respectively, the time of spawning management period and the yield of fruit body were detected. The result showed the treatment of adding microcrystalline cellulose (0.1%) or cellobiose (0.1%) to the liquid culture at I stage that 24 h before the time used as normal inoculants, which could shorten the spawning management period by three days, and increased the yield of first tidal mushroom by 4% and 11% respectively. The technology of shortening the spawning management period is constructed in this study, which could provide reference data for the oyster mushroom spawning bags producing enterprise in order to enhance the quality and increase the effect.
文章引用:潘戈帆, 许爱清. 木质纤维素酶诱导物对平菇菌包发菌期的影响[J]. 农业科学, 2023, 13(5): 391-399. https://doi.org/10.12677/HJAS.2023.135053

参考文献

[1] Royse, D.J., Baars, J. and Tan, Q. (2017) Current Overview of Mushroom Production in the World. In: Zied, D.C. and Pardo-Giménez, A., Eds., Edible and Medicinal Mushrooms: Technology and Applications, John Wiley & Sons Ltd. [Google Scholar] [CrossRef
[2] 谢君, 黄乾明, 冯蕾, 等. 白腐菌液体和固体培养产生木质纤维素降解酶的比较研究[J]. 菌物学报, 2007, 26(2): 266-272.
[3] Kumaran, S., Sastry, C.A. and Vikineswary, S. (1997) Laccase, Cellulase and Xylanase Activities during Growth of Pleurotus sajor-caju on Sago Hampas. World Journal of Microbiology & Biotechnology, 13, 43-49. [Google Scholar] [CrossRef
[4] 韩美玲, 安琪, 吴雪君, 等. 不同木质纤维素诱导对糙皮侧耳液体发酵产漆酶活性的影响[J]. 菌物学报, 2017, 36(3): 349-357.
[5] Corrêa, R.C.G., da Silva, B.P., Castoldi, R., et al. (2016) Spent Mushroom Substrate of Pleurotus pulmonarius: A Source of Easily Hydrolyzable Lignocellulose. Folia Microbiologica, 61, 439-448. [Google Scholar] [CrossRef] [PubMed]
[6] Obodai, M., Cleland-Okine, J. and Vowotor, K.A. (2003) Com-parative Study on the Growth and Yield of Pleurotus ostreatus Mushroom on Different Lignocellulosic By-Products. Journal of India Microbiology Biotechnology, 30, 146- 149. [Google Scholar] [CrossRef] [PubMed]
[7] 冯海萍, 杨冬艳, 谢华, 等. 农业生物质资源木质纤维素及基质化利用研究进展[J]. 贵州农业科学, 2017, 45(5): 144-147.
[8] 李登龙, 李明源, 王继莲, 等. 木质纤维素预处理方法研究进展[J]. 食品工业科技, 2019, 40(19): 326-332.
[9] 张飞, 白凤武, 赵心清. 丝状真菌纤维素酶合成诱导及转录调控[J]. 生物工程学报, 2016, 32(11): 1481-1495.
[10] Ariff, I.N.M., Bahrin, E.K., Ramli, N., et al. (2019) Direct Use of Spent Mushroom Substrate from Pleurotus pulmonarius as a Readily Delignified Feedstock for Cellulase Production. Waste and Biomass Valorization, 10, 839-850. [Google Scholar] [CrossRef
[11] 沈建, 孙凌燕, 奚菊红, 等. 纤维素水解液对鸡腿菇纤维素酶诱导特征[J]. 上海师范大学学报(自然科学版), 2007, 36(6): 63-67.
[12] 蔡英丽, 刘伟, 陈连福, 等. 香菇菌丝在不同碳源诱导下纤维素酶基因表达模式研究[C]//中国菌物学会. 中国菌物学会2015年学术年会论文摘要集: 2015年卷. 2015.
[13] 龚娜, 刘国丽, 陈珣, 等. 玉米芯对平菇菌丝体生长和胞外纤维素酶活性的影响研究[J]. 中国食用菌, 2019, 38(1): 41-44.