革兰阳性菌致脓毒症大鼠血清炎症因子浓度变化的研究

Study on the Change of Serum Inflammatory Factors Concentration in Septic Rats Caused by Gram-Positive Bacteria

摘要: 目的：研究革兰氏阳性细菌所致脓毒症大鼠血浆炎症因子的浓度变化，寻找脓毒症早期免疫干预的方式及最佳时机。方法：选取96只SD雄性大鼠，随机分组革兰氏阳性组(G⁺组，n = 48)、生理盐水组(NS组，n = 48)，分别腹腔注射热灭活金黄色葡萄球菌(7.35 × 10¹² CFU/Kg)、生理盐水，注射体积均为3 ml，造模成功后分别于2小时、4小时、6小时、8小时、12小时、24小时、48小时、72小时予以乙醚吸入麻醉革兰氏阳性大鼠6只及NS组大鼠6只，尾静脉采血，即刻离心机离心，取上清液用ELISA检测试剂盒检测各组大鼠血清TNF-α、IL-1β、IL-6、IL-10浓度。结果：G⁺组大鼠血清IL-10于2小时达到峰值，IL-1β、TNF-α于6小时达到峰值，IL-6于8小时达到峰值，各炎症因子与NS组对比差异具有统计学意义(P < 0.05)。NS组血清IL-10、IL-1β、IL-6、TNF-α从2小时开始变化不大，差异无统计学意义(P > 0.05)。结论：腹腔注射热灭活革兰阳性菌构建的脓毒症模型，2小时内开始免疫增强治疗，可能对脓毒症的治疗极具意义。

Abstract: Objective: To study the changes of plasma inflammatory factors in septic rats caused by Gram- posi-tive bacteria, and to find the way and the best opportunity for early immune intervention in sepsis. Methods: 96 SD male rats were randomly divided into Gram-positive group (G⁺ group, n = 48) and normal saline group (NS group, n = 48). Heat-inactivated Staphylococcus aureus (7.35 × 10¹² CFU/Kg) and normal saline were injected intraperitoneally respectively, and the injection volume was 3 ml. After successful modeling, 6 Gram-positive rats and 6 NS rats were anesthetized with ether at 2 hours, 4 hours, 6 hours, 8 hours, 12 hours, 24 hours, 48 hours and 72 hours respectively. The chest cavity was opened, blood was taken from the heart, and the supernatant was immediately centrifuged by centrifuge. The serum concentrations of TNF-α, IL-1β, IL-6 and IL-10 in each group were detected by ELISA detection kit. Results: Serum IL-10 in G⁺ group peaked at 2 hours, IL-1β and TNF-α peaked at 6 hours, and IL-6 peaked at 8 hours. The differences of inflammatory factors be-tween G+ group and NS group were statistically significant (P < 0.05). Serum IL-10, IL-1β, IL-6 and TNF-α in NS group changed little from 2 hours, and the difference was not statistically significant (P > 0.05). Conclusion: The sepsis model established by intraperitoneal injection of heat-inactivated Gram-positive bacteria can start immune enhancement therapy within 2 hours, which may be of great significance to the treatment of sepsis.