药用真菌杨树菇SUMO基因及抗肿瘤活性初步鉴定

The Molecular Cloning and Antitumor Activity Characterization of SUMO from Medicinal Fungus Agrocybe aegerita

摘要: 目的：克隆杨树菇抗肿瘤相关蛋白基因，初步鉴定其活性。方法：构建cDNA文库，随机测序得到SUMO (Small Ubiquitin-like Modifier)基因，通过构建真核表达质粒，并检测其在HeLa细胞中表达引起的细胞凋亡及衰老的变化，初步鉴定其抗肿瘤活性。结果：得到全长为606 bp的SUMO cDNA序列，编码103个氨基酸，将表达SUMO的真核表达质粒转染入HeLa细胞中，发现可引起肿瘤细胞的凋亡，但对细胞衰老无影响。结论：药用真菌中SUMO等小泛素类蛋白酶系统值得进一步研究，有助于新型抗肿瘤药物前体的开发。 Objective: The antitumor-associated gene of Agrocybe aegerita was cloned to identify the bioactivity. Method: cDNA library was constructed and the gene SUMO was obtained by random sequencing. The eukaryotic ex- pression vector was constructed to detect cell apoptosis and senescence activity. Result: The gene of SUMO was cloned with 606 bp and 103 amino acid residues. The expression in HeLa cells could induce the cell apoptosis instead of se- nescence. Conclusion: SUMO from medicinal fungus worth to be investigated for the discovery of new antitumor medicine.