壳寡糖对痛风性关节炎动物模型的治疗效果的研究

The Effect Study of Chitooligosaccharides on Acute Gouty Arthritis Animal Model

摘要: 目的：探讨海洋活性药物壳寡糖对尿酸钠晶体(msu)诱导的小鼠痛风性关节炎模型的治疗效果，并初步探讨其可能的作用机制。方法：选取健康的雄性WT野生型小鼠随机分成正常对照组、模型对照组、阳性药物组、壳寡糖(高1、高2、中、低剂量)组，每组6只小鼠，连续给药灌胃14天，第8天构建小鼠痛风性关节炎模型，观察壳寡糖对小鼠急性痛风性关节炎的关节周径、机械性超敏反应、关节红肿处的热痛觉的影响。结果：尿酸钠晶体可诱发小鼠急性痛风性关节炎，壳寡糖对尿酸钠晶体所致的关节处的红肿热痛等症状有抑制作用。结论：小鼠足垫内注射尿酸钠晶体可以成功诱导小鼠急性痛风性关节炎模型，壳寡糖在小鼠急性痛风性关节炎模型中可抑制急性痛风性关节炎的发作。

Abstract: Objective: To investigate the therapeutic effect of Marine active drug chitosan oligosaccharides on gouty arthritis in mice induced by sodium urate crystal (msu), and to explore its possible mecha-nism. Methods: Healthy male WT wild-type mice were randomly divided into normal control group, model control group, positive drug group, chitooligosaccharide (high dose 1, high dose 2, medium dose and low dose) group, with 6 mice in each group. The mice were treated with chitooligosaccha-ride by gavage for 14 days, and the gouty arthritis model was established on the 8th day. Observe the effects of chitooligosaccharides on the joint circumference, mechanical hypersensitivity, and thermal pain sensation in acute gouty arthritis in mice. Results: Sodium urate crystals could induce acute gouty arthritis in mice, and chitosan oligosaccharides could inhibit the symptoms such as redness, swelling, heat and pain in the joints caused by sodium urate crystals. Conclusion: Acute gouty arthritis can be successfully induced by injection of sodium urate crystals into the mouse an-kle cavity, and chitosan oligosaccharides can inhibit the onset of acute gouty arthritis in a mouse model.