Tubacin增敏在胱氨酸剥夺条件下三阴型乳腺癌铁死亡

Tubacin plus Erastin Treatment Sensitizes Cell Ferroptosis of Triple Negative Breast Neoplasms

摘要: 目的：Tubacin可以转变恶性肿瘤的胱氨酸不敏感的状态，我们研究主要目的是验证Tubacin在胱氨酸剥夺条件下处理三阴型乳腺癌细胞HCC38的实际效果，并验证细胞死亡类型是否为铁死亡。方法：将细胞株HCC38在DMSO、Tubacin、Erastin、及Tubacin联合Erastin四组条件下培养，通过7-AAD、LDH、ROS及脂质过氧化物检测细胞死亡及类型。结果：1) 在胱氨酸剥夺处理条件下加入Tubacin，HCC38细胞死亡增加；2) Tubacin联合Erastin处理HCC38，细胞死亡显著增加；3) Tubacin联合Erastin处理的HCC38，细胞脂质过氧化物显著和盘状结构域受体2增多。结论：Tubacin增敏在胱氨酸剥夺条件下三阴型乳腺癌铁死亡。

Abstract: Purpose: Tubain can change the cystine insensitive state of malignant tumors. Our main purpose is to verify the actual effect of Tubain on HCC38 cells of triple negative breast cancer under the condi-tion of cystine deprivation, and to verify whether the type of cell death is iron death. Methods: Cell line HCC38 was cultured under four conditions: DMSO, tubacin, erastin, and tubacin plus erastin, and cell death and types were determined by 7-AAD, LDH, ROS, and lipid peroxides. Results: 1) Ad-dition of tubacin under cystine deprivation treatment conditions increased HCC38 cell death; 2) Treatment of HCC38 with tubacin in combination with erastin significantly increased cell death; 3) HCC38, treated with tubacin in combination with erastin, showed a significant increase in cellular lipid peroxides and discoidin domain receptor 2. Conclusions: Tubacin sensitizes iron death in triple negative breast cancer under cystine deprived conditions.