原发性肝癌患者口腔菌群多样性研究

Study on the Diversity of Oral Microflora in Patients with Primary Hepatic Carcinoma

摘要: 目的：探讨口腔菌群与原发性肝癌的关系。方法：通过16S rRNA高通量测序技术分析10例原发性肝癌患者及10例健康体检者口腔菌群微生物多样性。结果：在门水平，与健康组相比肝癌组厚壁菌门丰度显著升高(P = 0.005)，变形菌门丰度显著降低(P = 0.041)。在属水平，肝癌组链球菌属丰度显著升高(P = 0.023)，梭杆菌属、拟普氏菌属、普氏菌属丰度显著降低(P = 0.019, P = 0.041, P = 0.028)。结论：原发性肝癌患者存在口腔菌群失调，其中链球菌属丰度显著升高，梭杆菌属、拟普氏菌属、普氏菌属丰度显著降低，能够作为生物标志物辅助原发性肝癌的早期诊断。

Abstract: Objective: To investigate the relationship between oral microflora and primary hepatic carcinoma. Methods: 16S rRNA high-throughput sequencing and bioinformatic analysis were used to examine oral bacterial diversity in the different patients including 10 patients with primary hepatic carci-noma, and 10 healthy controls. Results: At the phylum level, the proportion of Firmicutes was in-creased significantly (P = 0.005), however, the proportion of Proteobacteria was decreased signifi-cantly in L group (P = 0.041). At the genus level, the proportion of Streptococcus was increased sig-nificantly (P = 0.023), whereas the proportion of Fusobacterium, Alloprevotella and Prevotella de-creased significantly in L group (P = 0.019, P = 0.041, P = 0.028). Conclusion: The statistical analyses confirmed that there are significant differences between every two groups in one phylum and three genera, which could be used for biomarkers in diagnosis of primary hepatic carcinoma.