菌根菌接种对松柏类古树生长促生效应研究

Study on Promoting Effect of Mycorrhizal Inoculation on the Growth of Ancient Coniferous Trees

摘要: 目的：研究接种菌根菌对上海地区松柏类古树生长的影响，为应用菌根菌促生古树生长提供科学依据。方法：以上海地区5种松柏类古树(五针松Pinus parviflora、白皮松Pinus bungeana、黑松Pinus thunbergii、雪松Cedrus deodara、桧柏Sabina chinensis)为材料，采用南京林业大学提供的黄色须腹菌(Rhizopogon luteolus, R1)、多噬伯克霍尔德氏菌(Burkholderia multivorans, FJ9)、美味牛肝菌(Boletus edulis, Be)进行接种。结果：结果表明，接种1年后，各古树的生长量、叶绿素含量、根系活力和根系侵染率均有增加，尤其是根系活力增加明显，接种FJ9能够显著提升松柏古树的叶绿素含量，接种Rl、Be能够显著提升古树的菌根侵染率，每株(树)接种量10 L与15 L并无显著区别。结论：接种菌根菌对于松柏类古树的各项生理指标均有一定程度的促进作用，接种Rl、Be对于松柏类古树地下根系活力和菌根侵染率提升效果较好，接种FJ9对于叶绿素含量提升效果最好，但其中的机制尚需进一步研究。

Abstract: Objective: To study the effects of inoculation of mycorrhizal fungi on the growth of ancient coniferous trees in Shanghai, and to provide scientific basis for the application of mycorrhizal fungi to promote the growth of ancient trees. Methods: Five kinds of ancient coniferous trees (Pinus parviflora, Pinus bungeana, Pinus thunbergii, Cedrus deodara, and Sabina chinensis) and three mycorrhizal fungi (Rhizopogon luteolus, R1; Burkholderia multivorans, FJ9; Boletus edulis, Be) were used as materials. Results: The results showed that after one year after inoculation, the growth, chlorophyll content, root activity, and root colonization rate of all trees increased, especially the root activity increased significantly. Inoculation of FJ9 could significantly increase the chlorophyll content of ancient coniferous trees, inoculation of Rl and Be could significantly increase the mycorrhizal colonization rate of ancient trees, and there was no significant difference between inoculation amount of 10 L and 15 L. Conclusion: Inoculation of mycorrhizal fungi enhanced the physiological indexes of ancient coniferous trees to a certain extent. Inoculation of Rl and Be has a better effect on the root activity and mycorrhizal infection rate of ancient coniferous trees, while the inoculation of FJ9 has the best effect on the chlorophyll content, but the mechanism needs further study.