外泌体在创面愈合中的应用
Application of Exosomes in Wound Healing
DOI: 10.12677/ACM.2023.1361349, PDF,   
作者: 刘松梅, 王献珍*:青海大学附属医院,青海 西宁
关键词: 外泌体创面修复炎症介质Exosomes Wound Repair Inflammatory Mediators
摘要: 外泌体是内体衍生的囊泡,在细胞间通讯中起关键作用,分泌于多种生物液体中,包括血清、唾液、尿液、腹水和脑脊液等。外泌体很小(直径30~150 nm),具有独特的胆质蛋白结构,它们可以在细胞之间携带和交换各种物质。在此,我们对各种来源的外泌体在不同创面恢复过程中的最新应用进行综述,并简要概述在各种生物医学领域的应用现状。
Abstract: Exosomes are vesicles derived from endosomes, which play a key role in intercellular communica-tion and are secreted in various biological fluids, including serum, saliva, urine, ascites and cere-brospinal fluid. Exosomes are very small (30~150 nm in diameter) and have a unique bile protein structure, which can carry and exchange various substances between cells. Here, we review the lat-est application of exosomes from various sources in different wound healing processes, and briefly outline the application status in various biomedical fields.
文章引用:刘松梅, 王献珍. 外泌体在创面愈合中的应用[J]. 临床医学进展, 2023, 13(6): 9641-9647. https://doi.org/10.12677/ACM.2023.1361349

参考文献

[1] 李俊鹏, 李悦, 李晓鲁. 干细胞外泌体创面修复作用及机制研究进展[J]. 四川医学, 2023, 44(2): 191-194.
[2] Alenquer, M. and Amorim, M.J. (2015) Exosome Biogenesis, Regulation, and Function in Viral Infection. Viruses, 7, 5066-5083. [Google Scholar] [CrossRef] [PubMed]
[3] Yang, Q., Diamond, M.P. and Al-Hendy, A. (2016) The Emerging Role of Extracellular Vesicle-Derived miRNAs: Implication in Cancer Progression and Stem Cell Related Diseases. Journal of Clinical Epigenetics, 2, Article No. 13.
[4] Samanta, S., Rajasingh, S., Drosos, N., Zhou, Z., Dawn, B. and Rajasingh, J. (2018) Exosomes: New Molecular Targets of Diseases. Acta Pharmacologica Sinica, 39, 501-513. [Google Scholar] [CrossRef] [PubMed]
[5] Kalluri, R. and LeBleu, V.S. (2020) The Biology, Function, and Biomedical Applications of Exosomes. Science, 367, eaau6977. [Google Scholar] [CrossRef] [PubMed]
[6] 窦涵钰, 崔白苹, 丁小雷. 创面瘢痕形成机制研究进展[J]. 上海大学学报(自然科学版), 2022, 28(5): 831-840.
[7] Than, U.T.T., Guanzon, D., Leavesley, D. and Parker, T. (2017) Association of Extracellular Membrane Vesicles with Cutaneous Wound Healing. International Journal of Molecular Sciences, 18, Article No. 956. [Google Scholar] [CrossRef] [PubMed]
[8] Shou, J., Kong, X., Wang, X., et al. (2019) Tizoxanide Inhibits In-flammation in LPS-Activated RAW264.7 Macrophages via the Suppression of NF-κB and MAPK Activation. Inflam-mation, 42, 1336-1349. [Google Scholar] [CrossRef] [PubMed]
[9] 殷玉莲, 潘玲婷, 程亦勤, 陈红风. 巨噬细胞促进创面修复中作用的研究进展[J]. 海南医学院学报, 2019, 25(15): 1191-1195.
[10] Bian, D., Wu, Y., Song, G., Azizi, R. and Zamani, A. (2022) The Application of Mesenchymal Stromal Cells (MSCs) and Their Derivative Exosome in Skin Wound Healing: A Comprehensive Review. Stem Cell Research & Therapy, 13, Article No. 24. [Google Scholar] [CrossRef] [PubMed]
[11] An, Y., Lin, S., Tan, X., et al. (2021) Exosomes from Adi-pose-Derived Stem Cells and Application to Skin Wound Healing. Cell Proliferation, 54, e12993. [Google Scholar] [CrossRef] [PubMed]
[12] Shi, A., Li, J., Qiu, X., et al. (2021) TGF-β Loaded Exosome Enhances Is-chemic Wound Healing in Vitro and in Vivo. Theranostics, 11, 6616-6631. [Google Scholar] [CrossRef] [PubMed]
[13] Liu, J., Yan, Z., Yang, F., et al. (2021) Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Accelerate Cutaneous Wound Healing by Enhancing Angiogenesis through Delivering Angiopoietin-2. Stem Cell Reviews and Reports, 17, 305-317. [Google Scholar] [CrossRef] [PubMed]
[14] 唐强, 黄志群, 陆钢, 陈端凯, 姜艳, 唐乾利. 再生医疗技术对深II度烧伤患者的炎症因子水平及创面愈合的影响[J]. 中国美容医学, 2020, 29(4): 94-97.
[15] Bo, Y., Yang, L., Liu, B., et al. (2022) Exosomes from Human Induced Pluripotent Stem Cells-Derived Keratinocytes Accelerate Burn Wound Healing through miR-762 Mediated Promotion of Keratinocytes and Endothelial Cells Migration. Journal of Nanobiotechnology, 20, 291. [Google Scholar] [CrossRef] [PubMed]
[16] 新版《中国2型糖尿病防治指南》解读[J]. 临床荟萃, 2012(4): 357.
[17] Cuadros, D.F., Li, J., Musuka, G. and Awad, S.F. (2021) Spatial Epidemiolo-gy of Diabetes: Methods and Insights. World Journal of Diabetes, 12, 1042-1056. [Google Scholar] [CrossRef] [PubMed]
[18] Zhang, P., Lu, J., Jing, Y., Tang, S., Zhu, D. and Bi, Y. (2017) Glob-al Epidemiology of Diabetic Foot Ulceration: A Systematic Review and Meta-Analysis. Annals of Medicine, 49, 106-116. [Google Scholar] [CrossRef] [PubMed]
[19] Stoekenbroek, R.M., Lokin, J.L.C., Nielen, M.M., Stroes, E.S.G. and Koelemay, M.J.W. (2017) How Common Are Foot Problems among Individuals with Diabetes? Diabetic Foot Ulcers in the Dutch Population. Diabetologia, 60, 1271-1275. [Google Scholar] [CrossRef] [PubMed]
[20] Patel, S., Srivastava, S., Singh, M.R. and Singh, D. (2019) Mechanistic Insight into Diabetic Wounds: Pathogenesis, Molecular Targets and Treatment Strategies to Pace Wound Healing. Biomedicine & Pharmacotherapy, 112, Article ID: 108615. [Google Scholar] [CrossRef] [PubMed]
[21] Davis, F.M., Tsoi, L.C., Wasikowski, R., et al. (2020) Epige-netic Regulation of the PGE2 Pathway Modulates Macrophage Phenotype in Normal and Pathologic Wound Repair. JCI Insight, 5, e138443. [Google Scholar] [CrossRef] [PubMed]
[22] Kaushik, K. and Das, A. (2019) Endothelial Progenitor Cell Therapy for Chronic Wound Tissue Regeneration. Cytotherapy, 21, 1137-1150. [Google Scholar] [CrossRef] [PubMed]
[23] Li, J.H., Li, Y., Huang, D. and Yao, M. (2021) Role of Stromal Cell-Derived Factor-1 in Endothelial Progenitor Cell-Mediated Vascular Repair and Regeneration. Tissue Engineering and Regenerative Medicine, 18, 747-758. [Google Scholar] [CrossRef] [PubMed]
[24] 李罗成, 王志维, 吴红兵, 任宗力, 任伟. 内皮祖细胞来源的外泌体减轻血管内皮细胞缺氧性损伤[J]. 微循环学杂志, 2019, 29(4): 1-6.
[25] Li, P., Hong, G., Zhan, W., et al. (2023) Endothelial Progenitor Cell Derived Exosomes Mediated miR-182-5p Delivery Accelerate Diabetic Wound Heal-ing via Down-Regulating PPARG. International Journal of Medical Sciences, 20, 468-481. [Google Scholar] [CrossRef] [PubMed]
[26] Keshtkar, S., Azarpira, N. and Ghahremani, M.H. (2018) Mesenchymal Stem Cell-Derived Extracellular Vesicles: Novel Frontiers in Regenerative Medicine. Stem Cell Research & Therapy, 9, Article No. 63. [Google Scholar] [CrossRef] [PubMed]
[27] Shen, T., Zheng, Q.Q., Shen, J., et al. (2018) Effects of Adi-pose-Derived Mesenchymal Stem Cell Exosomes on Corneal Stromal Fibroblast Viability and Extracellular Matrix Syn-thesis. Chinese Medical Journal (England), 131, 704-712. [Google Scholar] [CrossRef] [PubMed]
[28] Wahlgren, J., Statello, L., Skogberg, G., Telemo, E. and Valadi, H. (2016) Delivery of Small Interfering RNAs to Cells via Exosomes. Methods in Molecular Biology, 1364, 105-125. [Google Scholar] [CrossRef] [PubMed]
[29] Bai, L., Shao, H., Wang, H., et al. (2017) Effects of Mesen-chymal Stem Cell-Derived Exosomes on Experimental Autoimmune Uveitis. Scientific Reports, 7, Article No. 4323. [Google Scholar] [CrossRef] [PubMed]
[30] Yu, B., Shao, H., Su, C., et al. (2016) Exosomes Derived from MSCs Ameliorate Retinal Laser Injury Partially by Inhibition of MCP-1. Scientific Reports, 6, Article No. 34562. [Google Scholar] [CrossRef] [PubMed]
[31] Han, K.Y., Tran, J.A., Chang, J.H., Azar, D.T., Zieske, J.D. (2017) Poten-tial Role of Corneal Epithelial Cell-Derived Exosomes in Corneal Wound Healing and Neovascularization. Scientific Re-ports, 7, Article No. 40548. [Google Scholar] [CrossRef] [PubMed]
[32] Zhang, J., Chen, C., Hu, B., et al. (2016) Exosomes Derived from Human Endothelial Progenitor Cells Accelerate Cutaneous Wound Healing by Promoting Angiogenesis through Erk1/2 Signaling. International Journal of Biological Sciences, 12, 1472-1487. [Google Scholar] [CrossRef] [PubMed]
[33] Zhang, B., Wang, M., Gong, A., et al. (2015) HucMSC-Exosome Mediated-Wnt4 Signaling Is Required for Cutaneous Wound Healing. Stem Cells, 33, 2158-2168. [Google Scholar] [CrossRef] [PubMed]
[34] Amariglio, N., Hirshberg, A., Scheithauer, B.W., et al. (2009) Donor-Derived Brain Tumor Following Neural Stem Cell Transplantation in an Ataxia Telangiectasia Patient. PLOS Medicine, 6, e1000029. [Google Scholar] [CrossRef] [PubMed]
[35] Herberts, C.A., Kwa, M.S. and Hermsen, H.P. (2011) Risk Factors in the Development of Stem Cell Therapy. Journal of Translational Medicine, 9, Article No. 29. [Google Scholar] [CrossRef] [PubMed]
[36] Jia, R., Li, J., Rui, C., et al. (2015) Comparative Proteomic Profile of the Human Umbilical Cord Blood Exosomes between Normal and Preeclampsia Pregnancies with High-Resolution Mass Spectrometry. Cellular Physiology and Biochemistry, 36, 2299-2306. [Google Scholar] [CrossRef] [PubMed]
[37] Hu, Y., Rao, S.S., Wang, Z.X., et al. (2018) Exosomes from Human Umbilical Cord Blood Accelerate Cutaneous Wound Healing through miR-21-3p-Mediated Promotion of Angiogenesis and Fibroblast Function. Theranostics, 8, 169-184. [Google Scholar] [CrossRef] [PubMed]
[38] Zhang, Y., Pan, Y., Liu, Y., et al. (2021) Exosomes Derived from Human Umbilical Cord Blood Mesenchymal Stem Cells Stimulate Regenerative Wound Healing via Transforming Growth Fac-tor-β Receptor Inhibition. Stem Cell Research & Therapy, 12, Article No. 434. [Google Scholar] [CrossRef] [PubMed]
[39] Li, C., Hou, X., Zhang, P., et al. (2020) Exosome-Based Tumor Therapy: Opportunities and Challenges. Current Drug Metabolism, 21, 339-351. [Google Scholar] [CrossRef] [PubMed]
[40] Soares Martins, T., Trindade, D., Vaz, M., et al. (2021) Diagnostic and Therapeutic Potential of Exosomes in Alzheimer’s Disease. Journal of Neurochemistry, 156, 162-181. [Google Scholar] [CrossRef] [PubMed]
[41] Li, J., Li, Y., Li, P., et al. (2022) Exosome Detection via Surface-Enhanced Raman Spectroscopy for Cancer Diagnosis. Acta Biomaterialia, 144, 1-14. [Google Scholar] [CrossRef] [PubMed]
[42] Liang, Y., Duan, L., Lu, J. and Xia, J. (2021) Engineering Exo-somes for Targeted Drug Delivery. Theranostics, 11, 3183-3195. [Google Scholar] [CrossRef] [PubMed]