2020年1~3月北京市朝阳区412例监测人群29种呼吸道多病原谱分析
Analysis on Results of 29 Respiratory Pathogens Nucleic Acid Detection among 412 Cases in Chaoyang District, Beijing from January to March 2020
摘要: 目的：了解2020年1~3月北京市朝阳区监测人群29种呼吸道病原感染情况。方法：采集412例监测人员呼吸道标本并提取核酸，使用实时荧光定量PCR同时检测29种呼吸道病原体。单因素分析使χ2检验或Fisher确切概率法，检验水准为α = 0.05，统计软件为SPSS 26.0。结果：412份标本中病原体阳性检出率62.38% (257/412)，其中细菌、病毒、非典型病原体阳性检出率分别为37.38% (154/412)、21.12% (87/412)、35.92% (148/412)。不同年龄段多病原体检出率具有统计学意义差异，99份标本显示混合感染24.03% (99/412)，非典型病原体合并细菌检出率57.58% (57/99)、新型冠状病毒合并其他病原体感染占44.44% (44/99)。结论：北京市朝阳区2020年1~3月412例监测人群29种呼吸道多病原谱以真菌、新型冠状病毒、金黄色葡萄球菌、流感嗜血杆菌、大肠杆菌为主；不同年龄段病原体检出率具有差异；混合感染多非典型病原体合并细菌为主。
Abstract: Objective: To investigate the spectrum of 29 respiratory pathogens in 412 cases of COVID-19 in Chaoyang District, Beijing from January to March in 2020. Methods: I. Nucleic acids were extracted from 412 samples and 29 respiratory pathogens were detected by real-time quantitative PCR. II. SPSS26.0 software was used for univariate analysis, χ2 test or Fisher exact probability method, and the test level was α = 0.05. Results: The detection results of 29 respiratory pathogens from 412 samples showed that the detection rate of positive samples was 62.38% (257/412). The detection rate of bacteria positive samples was 37.38% (154/412). The detection rate of virus positive sam-ples was 21.12% (87/412). The positive rate of atypical pathogens was 35.92% (148/412). There was no difference in the detection rate of multiple pathogens among different genders. The detec-tion rate of multiple pathogens in different age groups was statistically significant. The detection rate of mixed infection was 24.03% (99/412), and the detection rate of atypical pathogens with bacteria ranked the first, with 57.58% (57/99). 2019-nCoV was associated with other pathogens in 44.44% (44/99). Conclusion: There were 29 respiratory pathogens detected in 412 cases of COVID-19 in Chaoyang from January to March 2020, mainly fungi, 2019-nCoV, Staphylococcus aure-us, Haemophilus influenzae and Escherichia coli. The difference of detection rate of pathogens among age groups was significant. Mixed infections are mainly caused by atypical pathogens and bacteria.
文章引用：孙灵利, 宋衍燕, 李丽, 郝民, 顾琳, 张士尧. 2020年1~3月北京市朝阳区412例监测人群29种呼吸道多病原谱分析[J]. 医学诊断, 2023, 13(2): 139-145. https://doi.org/10.12677/MD.2023.132024

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