基于生信分析AMPK亚基在肾透明细胞癌中的作用
The Role of AMPK Subunits in Clear Cell Re-nal Cell Carcinoma Based on Biological In-formation Analysis
DOI: 10.12677/ACM.2023.1361381, PDF,    国家自然科学基金支持
作者: 刘尚奇, 胡艳波, 张慧明, 高 倩, 王敏杰*：内蒙古医科大学基础医学院，内蒙古 呼和浩特；高 峰, 杨 艳：内蒙古医科大学药学院，内蒙古 呼和浩特
关键词: TCGA；AMPK；亚基；肾透明细胞癌；TCGA； AMPK； Subunit； ccRCC
摘要: 目的：通过癌症基因组图谱(TCGA)数据评估AMPK亚基在肾透明细胞癌中的表达差异及患者生存预后相关性；同时探索其对免疫细胞浸润的影响。方法：使用TCGA的RNAseq数据和基因型组织表达(GTEx)数据库分析AMPK亚基的表达及预后价值。利用GeneCards数据库选取组成AMPK各个亚基的表达基因。使用R软件包survival (version3.2-7)进行AMPK的富集分析。我们从已发表的文章和在线数据库中下载了TCGA样本的免疫细胞浸润评分，并对免疫细胞浸润水平与AMPK表达之间的相关性进行了分析。结果：与健康人群相比，AMPK亚基在肾透明细胞癌组织中有显著差异表达。此外，AMPK各亚基表达与肾透明细胞癌预后，临床分期，免疫细胞浸润呈显著相关。结论：AMPK亚基的异常表达可能在肾透明细胞癌的发生发展中起重要作用，每个AMPK亚基可能与肾透明细胞癌有独立的相关性。
Abstract: Objective: Evaluate the differences in the expression differences of AMPK subunit in clear cell renal cell carcinoma through the cancer genome atlas (TCGA) data and patient survival prognosis correla-tion; simultaneously explore its impact on immune cell infiltration. Methods: Use TCGA’s RNAseq data and the genotype-tissue expression (GTEx) database to analyze the expression and prognosis value of AMPK subunits. Use the Gene Cards database to select an expression gene to form AMPK subunits. Use the R software package Survival (version3.2-7) for AMPK subunits enrichment. We downloaded the immune cell infiltration scores of the TCGA sample from published articles and online databases. We analyzed the correlation between the infiltration level of immune cells and the expression of AMPK subunits. Results: Compared with healthy people, AMPK subunits signifi-cantly differ in renal transparent cell carcinoma tissue. In addition, AMPK subunits expression is significantly correlated with the prognosis of renal evident cell cancer, clinical stage, and immune cell infiltration. Conclusion: The abnormal expression of the AMPK subunit may play an essential role in the development of clear cell renal cell carcinoma, and each AMPK subunit may have an in-dependent function.
文章引用：刘尚奇, 高峰, 杨艳, 胡艳波, 张慧明, 高倩, 王敏杰. 基于生信分析AMPK亚基在肾透明细胞癌中的作用[J]. 临床医学进展, 2023, 13(6): 9878-9885. https://doi.org/10.12677/ACM.2023.1361381

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