人脐带间充质干细胞的外泌体对肺癌细胞生物学活性的影响及其作用机制

Effect of Exosomes Derived from Human Um-bilical Cord Mesenchymal Stem Cells on Bio-logical Activity of Lung Cancer Cells and Its Mechanism

摘要: 目的：初步探究人脐带间充质干细胞来源的外泌体(Exosomes of human umbilical cord mesenchymal stem cells, hUCMSC-EXO)对非小细胞肺癌(non-small cell lung cancer, NSCLC)的影响以及其作用机制。方法：以A549细胞为研究模型构建荷瘤鼠，给予脐带间充质干细胞来源的hUCMSC-EXO治疗，并分别分为模型对照组、外泌体低剂量组、外泌体中剂量组、外泌体高剂量组和化疗组。分别从组织水平以肿瘤组织块的大小、组织形态、CD9的表达评价和细胞水平以细胞形态、增殖、分化进行评价，综合分析脐带间充质干细胞外泌体(hUCMSC-EXO)对NSCLC影响。结果：荷瘤鼠第8周用脊椎脱臼法处死，去掉皮肤剥离取出肿瘤组织测量其大小，统计分析各实验组的差异，结果显示模型对照组与外泌体低剂量组、中剂量组、高剂量组的差异具有统计学意义(P < 0.05)，对照组与化疗组的差异具有统计学意义(P < 0.05)，而外泌体低剂量组、中剂量组、高剂量组之间的差异没有统计学意义(P > 0.05)。结论：通过本研究证实：非小细胞肺癌A549细胞对hUCMSC-EXO反应具有剂量依赖性，高浓度的hUCMSC-EXO明显抑制肿瘤组织的生长和细胞迁移。

Abstract: Objective: To investigate the effect of human umbilical cord mesenchymal stem cell exosomes (hUCMSC-EXO) on non-small cell lung cancer (NSCLC) and its mechanism. Methods: A549 cells were used as the study model to construct tumor-bearing mice, which were treated with mesenchymal stem cell hUCMSC-EXO from umbilical cord, they were divided into model control group, low-dose exosome group, middle-dose exosome group, high-dose exosome group and chemotherapy group. The size, histomorphology, CD9 expression of tumor tissue and cell morphology, proliferation and differentiation of tumor tissue were evaluated at the tissue level, the effect of umbilical cord mes-enchymal stem cell exosomes (hUCMSC-EXO) on NSCLC was analyzed. Results: At the 8th week, the tumor-bearing mice were killed with the method of spinal dislocation, the size of the tumor tissue was measured after the skin was removed, and the difference among the experimental groups was statistically analyzed, the results showed that there were significant differences between model control group and exosome low-dose group, middle-dose group and high-dose group (P < 0.05), and control group and chemotherapy group (P < 0.05), there was no significant difference among low-dose, middle-dose and high-dose exosome groups (P > 0.05). Conclusion: This study confirmed that: The non-small-cell lung cancer A549 cells responded to hUCMSC-EXO in a dose-dependent manner, with high concentrations of hUCMSC-EXO significantly inhibiting tumor growth and cell migration.