基于网络药理学探究海巴戟天与PCOS的潜在作用研究

Exploring the Potential Effect Study of Morinda citrifolia and PCOS Based on Network Pharmacology

摘要: 目的：本文通过网络药理学等现代生物信息技术，初步探究海巴戟天在PCOS的潜在作用。方法：从中药系统药理学数据库与分析平台(TCMSP)数据库筛选出海巴戟天的有效活性成分及其对应的基因靶点；利用OMIM数据库检索出与PCOS发生发展相关的靶点；构建药物–疾病靶点韦恩图；利用STRING软件进行PCOS潜在的药物靶点的蛋白互作网络(PPI)，并通过DAVID数据库平台进行基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析。结果：筛选出海巴戟天的有效活性成分共有159种，对应的基因靶点有1022个；PCOS疾病相关基因靶点641个，药物–疾病的核心靶点有PIK3CD、MTOR、CDC42、SLC9A1、ODC1、EIF2AK3、CXCR4；GO功能分析及KEGG主要富集在钙介导的信号、细胞增殖的正向调控、蛋白丝氨酸/氨酸激酶活性等生物过程，以及白细胞经内皮迁移、甲状腺激素信号通路、非酒精性脂肪肝等信号通路。结论：基于生物信息学技术方法，海巴戟天可能作用于7个核心基因，并通过参与白细胞经内皮迁移、甲状腺激素信号通路等多种通路调控PCOS发生发展。

Abstract: Objective: This paper through modern bioinformation technology such as network pharmacology to explore the potential role of Morinda citrifolia in PCOS. Methods: The active ingredient and corre-sponding gene targets of Morinda citrifolia were screened from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The OMIM database was used to retrieve the targets related to the occurrence and development of PCOS, and the drug-disease tar-gets Venn diagram was constructed. Using STRING software to carry out Protein-Protein Interaction (PPI) of potential drug targets for PCOS, and gene ontology (GO) and Kyoto Encyclopedia of Genes of Genes and Genomes (KEGG) enrichment analysis through the DAVID database platform. Results: A total of 159 active ingredients were screened, and 1022 corresponding gene targets were screened. There are 641 PCOS disease-related gene targets, and the core targets of drug-disease are PIK3CD, MTOR, CDC42, SLC9A1, ODC1, EIF2AK3, CXCR4; GO function analysis and KEGG are mainly enriched in calcium-mediated signaling, positive regulation of cell proliferation, protein serine/aminokinase activity and other biological processes, as well as leukocyte endothelial migration, thyroid hormone signaling pathway, nonalcoholic fatty liver disease and other signaling pathways. Conclusion: Based on bioinformatics detection, Morinda citrifolia may act on 7 core genes and regulate the occurrence and development of PCOS by participating in various pathways such as leukocyte transendothelial migration and thyroid hormone signaling pathway.