磁珠纳米探针结合免疫层析法联级信号放大快速检测奶制品中的两种致病菌

Cascade Signal Amplification Rapid Detection of Two Pathogenic Bacteria in Dairy Products Using Magnetic Bead Nanoprobes Combined with Immunochromatographic

摘要: 阪崎氏肠杆菌和金黄色葡萄球菌是乳制品中致病率较高且常见的检测项目，本研究首先通过免疫磁分离技术实现目标菌的有效富集，然后通过新型的纳米荧光材料量子点及生物素–链霉亲和素体系结合免疫层析法实现阪崎氏肠杆菌和金黄色葡萄球菌联级信号放大和超灵敏联合检测。通过对研究整体的相关优化，两种菌检测灵敏度均可达到2 × 103 CFU/mL，相比传统的免疫层析法检测灵敏度提高了近2000倍。可实现同时超灵敏检测食源性致病菌的，为食品安全快速高灵敏准确检测大分子目标物提供了新的思路。

Abstract: Enterobacter sakazakii and Staphylococcus aureus are common detection items with high pathogenicity in dairy products. In this study, we first achieved effective enrichment of target bacteria through immunomagnetic separation, realized signal amplification and sensitive detection of Enterobacter sakazakii and Staphylococcus aureus through quantum dots and biotin streptavidin system combined with immunochromatography. By optimizing the overall research, both bacterial detection sensitivities can reach 2 × 103 CFU/mL, which increases the detection sensitivity by nearly 2000 times compared to traditional immunochromatography. It can achieve simultaneous sensitive detection of foodborne pathogens, providing a new approach for food safety to quickly, sensitively, and accurately detect large molecular targets.