p53、PTEN和MMR在子宫内膜癌中的表达及关联

Expression and Association of p53, PTEN and MMR in Endometrial Carcinoma

摘要: 目的：旨在分析53抑癌基因(p53)、张力蛋白同源物(PTEN)及错配修复蛋白(MMR)在子宫内膜癌(EC)组织中的表达，并探讨三种因子与EC临床病理特征的关系。方法：采用免疫组化方法检测90例正常子宫内膜、90例子宫内膜癌组织中p53、PTEN及MMR的表达水平，并分析三者间及与各项临床病理参数的关系。结果：1) EC组p53野生型表达率53.3% (48/90)、PTEN阳性表达率44.4% (40/90)，正常内膜组(NE组) 73.3% (66/90)、97.7% (88/90)低，P值分别为P = 0.005、P < 0.001，差异有统计学意义。2) 子宫内膜癌中p53及PTEN的表达与FIGO分期、组织学分级、深肌层浸润、脉管浸润、淋巴结转移有关，MMR表达与组织学分期有关。3) MSI与p53、PTEN的表达呈正相关(r = 0.213, P = 0.044; r = 0.209, P = 0.048)，PTEN与p53表达也呈正相关(r = 0.523, P < 0.001)。结论：PTEN、p53的表达及MMR缺失与EC患者病情发生发展及预后存在相关性，且p53、PTEN、MMR的表达存在正相关关系。

Abstract: Objective: To analyze the expression of 53 tumor suppressor gene (p53), phosphatase and tensin homolog (PTEN) and mismatch repair protein (MMR) in endometrial carcinoma (EC) tissues, and to explore the relationship between these three factors and the clinicopathologic features of EC. Methods: Immunohistochemical methods were used to detect the expression levels of p53, PTEN and MMR in 90 cases of normal endometrial and 90 cases of endometrial cancer, and to analyze the relationship between the three and various clinicopathological parameters Results: 1) The wild type expression rate of p53 was 53.3% (48/90) in EC group, and the positive expression rate of PTEN was 44.4% (40/90), while the normal endometrial group (NE group) was 73.3% (66/90) and 97.7% (88/90) lower, with P = 0.005 and P < 0.001, respectively, indicating statistically significant differences. 2) The expression of p53 and PTEN in endometrial carcinoma is related to FIGO stage, histological grade, deep muscle invasion, vascular invasion and lymph node metastasis, and the ex-pression of MMR is related to histological stage. 3) MSI was positively correlated with the expression of p53 and PTEN (r = 0.213, P = 0.044; r = 0.209, P = 0.048), PTEN was also positively correlated with p53 expression (r = 0.523, P < 0.001). Conclusion: The expression of PTEN and p53 and the absence of MMR were correlated with the development and prognosis of EC patients, and the ex-pression of p53, PTEN and MMR was positively correlated.