ANLN敲除对肺腺癌细胞在裸鼠体内生长的影响
Effect of ANLN Knockout on the Growth of Lung Adenocarcinoma Cells in Nude Mice
DOI: 10.12677/ACM.2023.1361454, PDF,    科研立项经费支持
作者: 朱思明*, 李 标#：海南医学院第二附属医院胸外科一区，海南 海口
关键词: ANLN；肿瘤；增殖及迁移；非小细胞肺癌；ANLN； Neoplasms； Proliferation and Migration； Non-Small Cell Lung Cancer
摘要: 目的：通过敲除肺腺癌(Lung adenocarcinoma, LUAD)细胞Anillin肌动蛋白结合蛋白(anillin actin binding protein, ANLN)基因，验证其对肺癌细胞增殖、迁移等生物功能及行为学影响。材料与方法：将裸鼠分为对照组和实验组，在对照组裸鼠中皮下注射对照组(Ctrl-A549)肺癌细胞悬液，在实验组裸鼠中注射实验组(ANLN-A549)肺癌细胞悬液，观察两组裸鼠皮下瘤体变化情况，记录瘤体长短径、瘤体重量并计算瘤体体积，整理数据，进行对比分析；用RT-PCR和Western Blot方法确定两组裸鼠成瘤的瘤体中ANLN基因的mRNA及蛋白表达情况。结果：裸鼠皮下成瘤实验表明，裸鼠皮下注射肺癌细胞(A549) 24天后，对照组及实验组中的裸鼠均有瘤体形成，成瘤率为100%。养殖观察期间，随着时间的延长，两组裸鼠皮下肿瘤的体积也不断增大，比较8个时间点的裸鼠皮下瘤体体积，得出实验组(ANLN-A549)相比较于对照组(Ctrl-A549)增大的速度更为缓慢，两组不同时间点瘤体体积大小均有明显差异(p < 0.05)；在最后时间节点，将小鼠进行安乐死后，取出皮下肿瘤瘤体并进行称重，结果显示实验组(ANLN-A549)瘤体重量较对照组(Ctrl-A549)明显减小(p < 0.05)。RT-PCR和Western Blot实验结果表明，实验组(ANLN-A549)中ANLN的mRNA及蛋白含量较对照组(Ctrl-A549)明显降低(p < 0.05)。结论：ANLN基因的敲减显著抑制肺腺癌细胞在裸鼠体内的增殖能力。
Abstract: Objective: To investigate the effect of Anillin actin binding protein (ANLN) gene on proliferation, migration and behavior of lung adenocarcinoma (LUAD) cells, the expression of ANLN gene in LUAD cells was knocked out. Materials and Methods: The Nude mice were divided into control group and experimental group, the lung cancer cell suspension of control group (Ctrl-A549) was injected sub-cutaneously in the control group, and the lung cancer cell suspension of experimental group (ANLN-A549) was injected subcutaneously in the experimental group. The changes of subcutaneous tumors in the two groups were observed, the length and weight of tumors were recorded, the vol-ume of tumors was calculated, and the data were collated and analyzed. The mRNA and protein ex-pression of ANLN gene in the tumors of the two groups were determined by RT-PCR and Western Blot. Results: The tumor formation rate was 100% in the control group and the experimental group after 24 days of subcutaneous injection of lung cancer cells (A549) in nude mice. During the obser-vation period, the subcutaneous tumor volume of the nude mice in the two groups increased with time, and the subcutaneous tumor volume of the nude mice in the experimental group (ANLN-A549) increased more slowly than that in the control group (Ctrl-A549) at 8 time points. There was a sig-nificant difference in tumor volume between the two groups at different time points (p < 0.05); at the last time point, the mice were euthanized, and the subcutaneous tumors were taken out and weighed. The results showed that the tumor weight of the experimental group (ANLN-A549) was significantly lower than that of the control group (Ctrl-A549) (p < 0.05). The results of RT-PCR and Western Blot showed that the mRNA and protein levels of ANLN in the experimental group (ANLN-A549) were significantly lower than those in the control group (Ctrl-A549) (p < 0.05). Con-clusion: Knockdown of ANLN gene significantly inhibits the proliferation of lung adenocarcinoma cells in nude mice.
文章引用：朱思明, 李标. ANLN敲除对肺腺癌细胞在裸鼠体内生长的影响[J]. 临床医学进展, 2023, 13(6): 10386-10394. https://doi.org/10.12677/ACM.2023.1361454

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