生物信息学方法分析动脉闭塞性疾病中的凋亡相关基因
Analysis of Apoptosis Related Genes in Arterial Occlusive Disease Using Bioinformatics Methods
摘要: 目的：动脉闭塞性疾病(arterial occlusive disease, AOD)是一种动脉疾病，其最常见的原因是动脉粥样硬化(atherosclerosis, AS)。凋亡相关基因在AOD的病理生理过程中起着至关重要的作用。因此，利用凋亡相关基因的表达谱来探讨凋亡与AOD之间的关系可能为其预防和治疗提供新的见解。方法：GSE57691数据集的差异表达的凋亡相关基因来自分子特征数据库(Molecular Signature Database, MSigDB)和基因表达(Gene Expression Omnibus, GEO)数据库，通过基因本体(Gene Ontology, GO)分析、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes, KEGG)、蛋白质互作网络(Protein-Protein Interaction Networks, PPI)分析GSE57691数据集的差异表达的凋亡相关基因。结果：基于GSE57691数据集，共选择了18个差异表达的凋亡相关基因。GO分析显示凋亡相关基因主要富集在胶质细胞发育及活化、生长因子受体结合等。KEGG分析表明凋亡相关基因主要富集在晚期糖基化终末产物(advanced glycation end products, AGE)与糖基化终末产物受体(receptor for advanced glycation end products, RAGE)信号通路糖尿病并发症、辅助性T细胞17 (T helper cell 17, Th17)细胞分化等。IL1B、IL6、SOD1、PPP3R1、APP等的表达被认为是PPI调控网络的重点。结论：凋亡和凋亡相关基因可能在调节AOD的病理生理学方面发挥重要作用。
Abstract: Objective: Arterial occlusive disease (AOD) is an arterial disease, the most common cause of which is atherosclerosis (AS). Apoptosis related genes play a crucial role in the pathophysiological process of AOD. Therefore, exploring the relationship between apoptosis and AOD using the expression profile of apoptosis related genes may provide new insights for its prevention and treatment. Method: The differentially expressed apoptosis related genes in GSE57691 data set came from Molecular Signa-ture Database (MSigDB) and Gene Expression Omnibus (GEO) database. The differentially ex-pressed apoptosis related genes in GSE57691 data set were analyzed through Gene Ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Protein-Protein Interaction Net-works (PPI). Result: Based on the GSE57691 dataset, a total of 18 differentially expressed apoptosis related genes were selected. GO analysis shows that apoptosis related genes are mainly enriched in the development and activation of glial cells, as well as the binding of growth factor receptors. KEGG analysis showed that apoptosis related genes are mainly enriched in the advanced glycation end products and receptor for advanced glycation end products (AGE-RAGE) signaling pathways diabet-ic complications, as well as in T helper cell 17 (Th17) cell differentiation. The expression of IL1B, IL6, SOD1, PPP3R1, APP, etc. is considered key focus of the PPI regulatory network. Conclusion: Apopto-sis and apoptosis related genes may play an important role in regulating the pathophysiology of AOD and AS.
文章引用：韩舒宁, 张翌帆. 生物信息学方法分析动脉闭塞性疾病中的凋亡相关基因[J]. 临床医学进展, 2023, 13(6): 10413-10420. https://doi.org/10.12677/ACM.2023.1361458

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