荧光光谱法在水质检测中的研究与应用进展
Research and Application Progress in Water Quality Detection by Fluorescence Spectrometry
DOI: 10.12677/JWRR.2023.123027, PDF,    科研立项经费支持
作者: 谢 琳, 赵明富, 汤 斌：重庆理工大学现代光电检测技术与仪器重点实验室，重庆；姜赞成：重庆大学光电技术及系统教育部重点实验室，重庆；杨江林*, 夏铭涌, 昝佳欣, 孙嘉怡：重庆市清华中学，重庆
关键词: 三维荧光光谱；数据分析；溶解有机物；水质检测；Three-Dimensional Fluorescence Spectrum； Data Analysis； Dissolved Organic Matter (DOM)； Water Quality Detection
摘要: 光谱检测法是近几年广受关注的一种水质分析方式，因其检测速度快、检测结果较为准确且不易造成二次污染受到了水质监测行业的大力推崇。文章中选取了光谱法中的荧光光谱法进行研究，介绍了荧光光谱检测原理及常用的荧光光谱谱图数据分析方法，探讨了荧光光谱法用于分析水体溶解有机物(Dissolved Organic Matter, DOM)的来源和特征的可行性，论述了目前在常规水质指标检测中荧光光谱的研究和应用进展。
Abstract: Spectral method is a kind of water quality analysis method which has been widely concerned in recent years. It is highly respected by the industry of water quality monitoring because of its fast detection speed, accurate detection results and not easy to cause secondary pollution. In this paper, fluorescence spectroscopy was selected for research, the principle of fluorescence spectroscopy detection and the commonly used fluorescence spectrogram data analysis methods were introduced, the feasibility of fluorescence spectroscopy for analyzing the source and characteristics of dissolved organic matter in water was discussed, and the current research and application progress of fluorescence spectroscopy in conventional water quality index detection was discussed.
文章引用：谢琳, 姜赞成, 赵明富, 杨江林, 夏铭涌, 昝佳欣, 孙嘉怡, 汤斌. 荧光光谱法在水质检测中的研究与应用进展[J]. 水资源研究, 2023, 12(3): 238-245. https://doi.org/10.12677/JWRR.2023.123027

参考文献

[1] 刘凌妃. 水质有机污染物的三维荧光光谱定性定量分析[D]: [硕士学位论文]. 秦皇岛: 燕山大学, 2018. LIU Lingfei. Qualitative and quantitative analysis of organic pollutants in water by three-dimensional fluorescence spectrum. Master’s Thesis, Qinhuangdao: Yanshan University, 2018. (in Chinese)
[2] 侯迪波, 张坚, 陈泠, 黄平捷, 张光新. 基于紫外-可见光光谱的水质分析方法研究进展与应用[J]. 光谱学与光谱分析, 2013, 33(7): 1839-1844. HOU Dibo, ZHANG Jian, CHEN Ling, HUANG Pingjie and ZHANG Guangxin. Research progress and application of water quality analysis methods based on UV-visible spectrum. Spectroscopy and Spectral Analysis, 2013, 33(7): 1839-1844. (in Chinese)
[3] STOREY, M. V., Van Der GAAG, B. and BURNS, B. P. Advances in on-line drinking water quality monitoring and early warning systems. Water Research, 2011, 45(2): 741-747. [Google Scholar] [CrossRef] [PubMed]
[4] 邰玲, 弓巧娟, 孙鸿, 张稳婵, 李华静. 荧光光谱法在水质监测中的应用[J]. 光谱实验室, 2011, 28(2): 940-945. TAI Ling, GONG Qiaojuan, SUN Hong, ZHANG Wenchan and LI Huajing. Application of fluorescence spectrometry in water quality monitoring. Laboratory of Spectroscopy, 2011, 28(2): 940-945. (in Chinese)
[5] 刘丽贞, 黄琪, 吴永明, 吴代赦, 游海林. 鄱阳湖CDOM三维荧光光谱的平行因子分析[J]. 中国环境科学, 2018, 38(1): 293-302. LIU Lizhen, HUANG Qi, WU Yongming, WU Daishe and YOU Hailin. CDOM Poyang Lake, the three-dimensional fluorescence spectrum parallel factor analysis. China Environmental Science, 2018, 38(1): 293-302. (in Chinese) [Google Scholar] [CrossRef] 
[6] 王迪, 张飞, 张兆永, 张海威, 朱世丹, 张贤龙. 新疆艾比湖流域枯、丰水期三维荧光光谱特性及其与水质的关系[J]. 湖泊科学, 2020, 32(2): 483-495. WANG Di, ZHANG Fei, ZHANG Zhaoyong, ZHANG Haiwei, ZHU Shidan and ZHANG Xianlong. Characteristics of three-dimensional fluorescence spectrum and its relationship with water quality during dry and wet seasons in Aibi Lake Basin, Xinjiang. Journal of Lake Sciences, 2010, 32(2): 483-495. (in Chinese)
[7] 汪之睿, 于静洁, 王少坡, 聂英进, 张轶凡. 三维荧光技术在水环境监测中的应用研究进展[J]. 化工环保, 2020, 40(2): 125-130. WANG Zhirui, YU Jingjie, WANG Shaopo, NIE Yingjin and ZHANG Yifan. Application of three-dimensional fluorescence technology in water environment monitoring. Environmental Protection of Chemical Industry, 20, 40(2): 125-130. (in Chinese)
[8] CHEN, W., WESTERHOFF, P., LEENHEER, J. A. and BOOKSH, K. Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter. Environmental Science & Technology, 2003, 37(24): 5701-5710. [Google Scholar] [CrossRef] [PubMed]
[9] 卢松, 江韬, 张进忠, 闫金龙, 王定勇, 魏世强, 梁俭, 高洁. 两个水库型湖泊中溶解性有机质三维荧光特征差异[J]. 中国环境科学, 2015, 35(2): 516-523. LU Song, JIANG Tao, ZHANG Jinzhong, YAN Jinlong, WANG Dingyong, WEI Shiqiang, LIANG Jian and GAO Jie. Difference of three-dimensional fluorescence characteristics of dissolved organic matter in two reservoir-type lakes. China Environmental Science, 2015, 35(2): 516-523. (in Chinese)
[10] CORY, R. M., MCKNIGHT, D. M. Fluorescence spectroscopy reveals ubiquitous presence of oxidized and reduced quinones in dissolved organic matter. Environmental Science & Technology, 2005, 39(21): 8142-8149. [Google Scholar] [CrossRef] [PubMed]
[11] 何杰, 李学艳, 林欣, 周飞, 汤如涛, 许法标. 光谱特征法辨识不同污染景观河道中溶解性有机物的组分与来源[J]. 环境科学学报, 2021, 41(3): 1000-1010. HE Jie, LI Xueyan, LIN Xin, ZHOU Fei, TANG Rutao and XU Fabiao. Identification of dissolved organic compounds in different polluted landscape watercourses by spectral characteristics. Journal of Environmental Science, 2021, 9(3): 1000-1010. (in Chinese) [Google Scholar] [CrossRef] 
[12] ZHOU, Y. Q., SHI, K., ZHANG, Y. L., JEPPESEN, E., LIU, X. H., ZHOU, Q. C., WU, H. W., TANG, X. M. and ZHU, G. W. Fluorescence peak integration ratio IC:IT as a new potential indicator tracing the compositional changes in chromophoric dissolved organic matter. Science of the Total Environment, 2017, 574: 1588-1598.[CrossRef] [PubMed]
[13] BAGHOTH, S. A., SHARMA, S. K. and AMY, G. L. Tracking natural organic matter (NOM) in a drinking water treatment plant using fluorescence excitation-emission matrices and PARAFAC. Water Research, 2011, 45(2): 797-809. [Google Scholar] [CrossRef] [PubMed]
[14] 祝鹏, 华祖林, 李惠民. 湖泊水体三维荧光光谱的PARAFAC法在污染源解析中的应用[J]. 光谱学与光谱分析, 2012, 32(12): 3290-3294. ZHU Peng, HUA Zulin and LI Huimin. Application of PARAFAC method of three-dimensional fluorescence spectrum in lake water source analysis. Spectroscopy and Spectral Analysis, 2012, 32(12): 3290-3294. (in Chinese)
[15] 周石磊, 孙悦, 张艺冉, 战江, 王函, 黄廷林, 丛海兵, 崔建升, 李再兴. 山东省周村水库季节演替中沉积物上覆水溶解性有机物的紫外-可见与三维荧光光学特征[J]. 湖泊科学, 2019, 31(5): 1344-1356. ZHOU Shilei, SUN Yue, ZHANG Yilan, ZHAN Jiang, WANG Han, HUANG Tinglin, CONG Haibing, CUI Jiansheng and LI Zaixing. Ultraviolet-visible and three-dimensional fluorescence optical characteristics of dissolved organic matter in sediment overhanging water during seasonal succession in Zhoucun Reservoir, Shandong Province. Journal of Lake Sciences, 2019, 31(5): 1344-1356. (in Chinese)
[16] OHNO, T., FERNANDEZ, I. J., HIRADATE, S. and SHERMAN, J. F. Effects of soil acidification and forest type on water soluble soil organic matter properties. Geoderma, 2007, 140(1): 176-187. [Google Scholar] [CrossRef] 
[17] ZSOLNAY, A., BAIGAR, E., JIMENEZ, M., STEINWEG, B. and SACCOMANDI, F. Differentiating with fluorescence spectroscopy the sources of dissolved organic matter in soils subjected to drying. Chemosphere, 1999, 38(1): 45-50. [Google Scholar] [CrossRef] 
[18] FAN, Z. H., GONG, S., XU, X., ZHANG, X. H., ZHANG, Y. and YU, X. Characterization, DBPs formation, and mutagenicity of different organic matter fractions in two source waters. International Journal of Hygiene and Environmental Health, 2014, 217(2-3): 300-306. [Google Scholar] [CrossRef] [PubMed]
[19] LU, X. W., ZHU, G. C. and WU, Y. F. Characteristics of DOM and removal of DBPs precursors across O3-BAC integrated treatment for the micro-polluted raw water of the Huangpu River. Water, 2013, 5(4): 1472-1486. [Google Scholar] [CrossRef] 
[20] KRASNER, S. W., WESTERHOFF, P., CHEN, B. Y., RITTMANN, B. E., NAM, S.-N. and AMY, G. Impact of wastewater treatment processes on organic carbon, organic nitrogen, and DBP precursors in effluent organic matter. Environmental Science & Technology, 2009, 43(8): 2911-2918. [Google Scholar] [CrossRef] [PubMed]
[21] XU, H., YAN, Z., CAI, H., et al. Heterogeneity in metal binding by individual fluorescent components in a eutrophic algae-rich lake. Ecotoxicology & Environmental Safety, 2013, 98: 266-272.[CrossRef] [PubMed]
[22] CADONDON, J. G., NAPAL, J. P. D., ABE, K., DE LARA, R., VALLAR, E. A., ORBECIDO, A. H., BELO, L. P. and GALVEZ, M. C. D. Characterization of water quality and fluorescence measurements of dissolved organic matter in Cabuyao river and its tributaries using excitation-emission matrix spectroscopy. Journal of Physics: Conference Series, 2020, 1593(1): 012033. [Google Scholar] [CrossRef] 
[23] 白璐, 徐雄, 刘权震, 杜艳君, 王东红. 武汉市不同类型天然水体中溶解性有机质的三维荧光光谱特征[J]. 光谱学与光谱分析, 2022, 42(5): 1642-1647. BAI Lu, XU Xiong, LIU Quanzhen, DU Yanjun and WANG Donghong. Three-dimensional fluorescence spectra of dissolved organic matter in different types of natural water bodies in Wuhan. Spectroscopy and Spectral Analysis, 202, 42(5): 1642-1647. (in Chinese)
[24] 杨欣, 吴支行, 叶寅, 陈晓芳, 袁自然, 王静. 店埠河农业小流域水体溶解性有机质三维荧光光谱的平行因子分析[J]. 光谱学与光谱分析, 2022, 42(3): 978-983. YANG Xin, WU Zhibian, YE Yin, CHEN Xiaofang, YUAN Zan and WANG Jing. Parallel factor analysis of three-dimensional fluorescence spectra of dissolved organic matter in Dianbu River Basin. Spectroscopy and Spectral Analysis, 2022, 42(3): 978-983. (in Chinese)
[25] 戴源, 谢继征, 袁静, 沈薇, 郭宏达, 孙小平, 王志刚. 三维荧光光谱技术结合线性支持向量算法在水体有机污染监测中的应用[J]. 光谱学与光谱分析, 2021, 41(9): 2839-2845. DAI Yuan, XIE Jizheng, YUAN Jing, SHEN Wei, GUO Hongda, SUN Xiaoping and WANG Zhigang. Application of three-dimensional fluorescence spectroscopy and linear support vector algorithm in organic pollution monitoring of water bodies. Spectroscopy and Spectral Analysis, 201, 41(9): 2839-2845. (in Chinese)
[26] 周铭睿, 曲江北, 李彭, 何义亮. 分散式农村污水基于三维荧光光谱和紫外-可见全波段吸收光谱的“聚类-回归” COD预测模型[J]. 光谱学与光谱分析, 2022, 42(7): 2113-2119. ZHOU Mingrui, QU Jiangbei, LI Peng and HE Yiliang. A clustering and regression model for COD prediction of distributed rural sewage based on three-dimensional fluorescence spectrum and UV-visible absorption spectrum. Spectroscopy and Spectral Analysis, 2022, 42(7): 2113-2119. (in Chinese)
[27] 周昆鹏, 白旭芳, 毕卫红. 荧光光谱法检测水质COD时温度、浊度、pH的影响分析[J]. 光谱学与光谱分析, 2019, 39(4): 1097-1102. ZHOU Kunpeng, BAI Xufang and BI Weihong. Analysis on the Influence of temperature, turbidity and pH in the determination of COD by Fluorescence Spectrometry. Spectroscopy and Spectral Analysis, 2019, 39(4): 1097-1102. (in Chinese)
[28] ZHAO, N. J., ZHANG, X. L., YIN, G. F., YANG, R. F., HU, L., CHEN, S., LIU, J. G. and LIU, W. Q. On-line analysis of algae in water by discrete three-dimensional fluorescence spectroscopy. Optics Express, 2018, 26(6): A251-A259. [Google Scholar] [CrossRef] 
[29] TANG, J. F., LI, X. H., CAO, C. L., LIN, M. X., QIU, Q. L. L., XU, Y. Y. and REN, Y. Compositional variety of dissolved organic matter and its correlation with water quality in peri-urban and urban river watersheds. Ecological Indicators, 2019, 104: 459-469.[CrossRef]