TLR4多态性与NAFLD易感性的相关性
Association between TLR4 Polymorphism and NAFLD Susceptibility
DOI: 10.12677/ACM.2023.1371508, PDF,   
作者: 赵守林, 宣世英*：青岛大学附属青岛市市立医院感染性疾病科，山东 青岛；赵真真：青岛大学附属青岛市市立医院临床研究中心，山东 青岛
关键词: 非酒精性脂肪性肝病；Toll样受体4；单核苷酸多态性；Nonalcoholic Fatty Liver Disease； Toll-Like Receptor 4； Single Nucleotide Polymorphism
摘要: 目的：探究中国青岛地区人群Toll样受体4 (Toll-like receptors 4, TLR4) rs1927914位点多态性与非酒精性脂肪性肝病(non-alcoholic fatty liver disease, NAFLD)易感性的相关性。方法：纳入2020年12月~2022年06月青岛市市立医院收入院的NAFLD患者218例，健康对照者101例。提取受试者血液中的DNA，使用多聚酶链反应(polymerase chain reaction, PCR)的方法扩增DNA，并检测TLR4基因rs1927914位点的基因型。收集并分析患者的与脂质代谢及肝脏代谢状态相关的指标。使用χ2检验分析基因型及等位基因频率。符合正态分布的计量资料采用t检验，不符合正态分布的计量资料采用Wilcoxon秩和检验进行组间比较。结果：NAFLD组和对照组TLR4 rs1927914位点的基因型与等位基因分布差异均无统计学意义。结论：在青岛地区人群中，TLR4 rs1927914位点多态性与NAFLD的无明显相关性。
Abstract: Objective: To explore the polymorphism of Toll-like receptors 4 (TLR4) rs1927914 locus and non- alcoholic fatty liver disease in Qingdao population of China association with susceptibility to NAFLD. Methods: 218 patients with NAFLD and 101 healthy controls admitted to Qingdao Municipal Hospi-tal from December 2020 to June 2022 were included. DNA was extracted from the subjects’ blood, amplified using polymerase chain reaction (PCR), and genotypes at rs1927914 locus of the TLR4 gene were detected. Indicators related to lipid metabolism and liver metabolic status were collect-ed and analyzed. Genotype and allele frequency were analyzed using χ2 test. T-test was used for measurement data conforming to normal distribution, and Wilcoxon rank sum test was used for comparison between groups for measurement data not conforming to normal distribution. Results: There were no significant differences in genotype and allele distribution of TLR4 rs1927914 be-tween NAFLD group and control group. Conclusion: There is no significant correlation between TLR4 rs1927914 polymorphism and NAFLD in Qingdao population.
文章引用：赵守林, 赵真真, 宣世英. TLR4多态性与NAFLD易感性的相关性[J]. 临床医学进展, 2023, 13(7): 10796-10802. https://doi.org/10.12677/ACM.2023.1371508

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