两株耐盐假单胞菌对盐胁迫下油菜种子萌发的影响

Effects of Two Salt Tolerant Pseudomonas Strains on the Germination of Rapeseed Seeds under Salt Stress

摘要: 为了探究植物根际促生菌对盐胁迫下油菜种子萌发的影响，本研究以甘蓝型油菜种子为研究对象，利用源于自贡盐矿区植物根际盐渍土中耐盐细菌韩国假单胞菌(Pseudomonas koreensis)和维罗尼假单胞菌(Pseudomonas veronii)的菌悬液浸染油菜种子，在不同浓度的NaCl (0、50、100和200 mmol/L)胁迫下进行纸上芽床发芽试验。结果显示，在不同浓度NaCl胁迫下，ST29和ST38菌悬液都不同程度地提高了油菜种子萌发的发芽势、发芽率和发芽指数，同时这两个菌株分别对油菜胚根和胚芽的生长都具有促进作用，其中ST38对油菜胚根和胚芽的促进生长要高于ST29的促进作用。以上结果表明，植物根际耐盐促生菌韩国假单胞菌和维罗尼假单胞菌能够提高油菜在盐胁迫下的萌发能力和协助油菜萌发后的抗逆促生能力。

Abstract: In order to investigate the effect of plant rhizosphere growth promoting bacteria on the germination of rapeseed seeds under salt stress, the germination of rapeseed seeds, which were soaked with the plant rhizosphere Pseudomonas koreensis and Pseudomonas veronii in the saline soil from the Zigong salt mining area, was studied on the paper sprout bed under different concentrations of NaCl (0, 50, 100, and 200 mmol/L) stress. The results showed that both ST29 and ST38 bacterial suspensions could increase the germination potential, germination rate, and germination index of rapeseed seeds under different concentrations of NaCl stress. At the same time, these two strains promoted the growth of rapeseed radicle and embryo, and ST38 had a higher promoting effect on the growth of rapeseed radicle and embryo than ST29. From above, it indicated that plant rhizosphere salt tolerant bacteria could improve the germination ability of rapeseed under salt stress and help to enhance the stress resistance and to promote the growth ability of rapeseed after germination.