黄连药材及其配方颗粒的薄层色谱鉴别研究

Study on TLC Identification of Coptis chinensis and Its Formula Granules

摘要: 目的：建立不同批次黄连药材及其配方颗粒的薄层鉴别方法。方法：以盐酸小檗碱为对照。采用硅胶G薄层板，以环己烷–乙酸乙酯–异丙醇–甲醇–水–三乙胺(2:4:1:1.5:0.5:2)为展开剂，置紫外光灯(365 nm)下检视。结果：优化后的薄层色谱鉴别方法斑点显示清晰，分离度好。结论：该方法可以用于黄连药材及其配方颗粒的的薄层鉴别。

Abstract: Objective: To establish a TLC identification method for different batches of Coptis chinensis and their formula granules. Methods: Berberine hydrochloride was used as control. Using a silica gel G thin layer plate, cyclohexane-ethyl acetate-isopropanol-methanol-water-triethylamine (2:4:1:1.5: 0.5:2) was used as the developing agent and examined under ultraviolet light (365 nm). Results: The optimized TLC identification method has clear spot display and good resolution. Conclusion: This method can be used for the TLC identification of Coptis chinensis medicinal herbs and their formula granules.