基于网络药理学探究桑叶–石榴皮复配抗皮肤糖化作用机制

Anti-Skin Glycosylation Mechanism of Folium Mori et Granati Pericarpium Formula Based on Network Pharmacology

摘要: 基于网络药理学对桑叶–石榴皮复配抗皮肤糖化作用机制进行初步探讨。通过TCMSP数据库收集桑叶、石榴皮活性成分及靶点。基于STRING、Metascape数据库进行蛋白互作、基因功能富集及信号通路分析。利用Cytoscape 3.7.1软件，构建“植物–活性成分–靶点”网络和PPI网络，找出核心成分和关键靶点。筛选得到槲皮素、山奈酚、花生四烯酸等5个核心成分，AKT1、VEGFA、IL6等10个关键靶点。KEGG分析显示，桑叶–石榴皮复配可作用于癌症、糖尿病并发症中的AGE-RAGE、胰岛素抵抗等多个代谢途径。运用网络药理学为系统阐述桑叶–石榴皮复配抗皮肤糖化作用机制提供了数据支持和科学依据。

Abstract: Based on network pharmacology, the mechanism of anti-skin glycation of Folium Mori et Granati Pericarpium formula was preliminarily discussed. The active components of Folium Mori et Granati Pericarpium and their targets were collected through TCMSP database. Based on STRING and Metascape databases, protein interaction, gene function enrichment and signaling pathway analysis were performed. Using Cytoscape 3.7.1 software, the “plant-active ingredient-target” network and PPI network were built to identify core targets and components. Five core components including quercetin, kaempferol and arachidonic acid and 10 key targets such as AKT1, VEGFA, and IL6 were screened. KEGG analysis showed that Folium Mori et Granati Pericarpium formula can act on multiple metabolic pathways such as cancer, AGE-RAGE signaling pathway in diabetic complications, and insulin resistance. Network pharmacology provides data support and scientific basis for the systematic elaboration of the anti-skin glycation mechanism of Folium Mori et Granati Pericarpium formula.