ALDH1A1在肝细胞癌中的表达及临床诊断价值探讨
The Expression and Clinical Diagnostic Value of ALDH1A1 in Hepatocellular Carcinoma
DOI: 10.12677/ACM.2023.1371662, PDF,    科研立项经费支持
作者: 刘 瑞, 陈元堃, 杨仪君, 陈贤珍, 刘佩云, 张福威：海南医学院第二附属医院感染病与热带病科，海南 海口；戢楠楠：海南医学院第二附属医院肿瘤放射治疗科，海南 海口；许德栋*：海南医学院第二附属医院神经外科，海南 海口
关键词: 乙醛脱氢酶1A1；肝细胞癌；临床；诊断；ALDH1A1； HCC； Clinical； Diagnosis
摘要: 目的：分析乙醛脱氢酶1A1 (ALDH1A1)在肝细胞癌(HCC)与正常肝脏中的表达差异，探讨ALDH1A1在HCC诊断中的价值。方法：实时荧光定量PCR (qRT-PCR)和免疫印迹法(Western blot)分别检测人肝癌细胞株HepG2与人正常肝细胞株LO2中ALDH1A1 mRNA及蛋白的表达情况；免疫组织化学法(IHC)检测49例HCC及其癌旁组织中ALDH1A1的表达情况。结果：人肝癌细胞株HepG2中ALDH1A1 mRNA及蛋白的表达均明显高于人正常肝细胞株LO2 (P < 0.05, P < 0.0001)；HCC组织中ALDH1A1的表达明显高于癌旁组织(P < 0.05)。结论：ALDH1A1在HCC中的表达升高，ALDH1A1有望作为诊断HCC的有效分子标志物。
Abstract: Objective: To analyze the differential expression of acetaldehyde dehydrogenase 1A1 (ALDH1A1) in Hepatocellular carcinoma (HCC) and normal liver, and to explore the value of ALDH1A1 in the diag-nosis of HCC. Methods: qRT PCR and Western blot were used to detect the expression of ALDH1A1 mRNA and protein in HepG2 and LO2, respectively; IHC was used to detect the expression of ALDH1A1 in 49 HCC and paracancerous tissues. Results: The expression of ALDH1A1 mRNA and protein in HepG2 was significantly higher than that in LO2 (P < 0.05, P < 0.0001); The expression of ALDH1A1 in HCC tissue was significantly higher than that in paracancerous tissues (P < 0.05). Con-clusion: The expression of ALDH1A1 is elevated in HCC, and ALDH1A1 is expected to serve as an ef-fective molecular marker for the diagnosis of HCC.
文章引用：刘瑞, 陈元堃, 戢楠楠, 杨仪君, 陈贤珍, 刘佩云, 张福威, 许德栋. ALDH1A1在肝细胞癌中的表达及临床诊断价值探讨[J]. 临床医学进展, 2023, 13(7): 11864-11869. https://doi.org/10.12677/ACM.2023.1371662

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