茜草药材及其配方颗粒TLC及HPLC指纹图谱研究

Study on TLC and HPLC fingerprint of Rubia cordifolia and Its Formula Granules

摘要: 目的：建立茜草药材及其配方颗粒的薄层色谱(TLC)和高效液相(HPLC)指纹图谱。方法：TLC法：展开剂为石油醚–丙酮(4:1.3)，在365 nm紫外光灯下检视。HPLC法：色谱柱为Diasonsil C18 (4.6 mm × 250 mm)，流动相为甲醇(A)-0.1%的磷酸水(B)，梯度洗脱，进样量10 μL，柱温30℃，体积流量1.0 mL/min。然后结合中药色谱指纹图谱相似度评价软件(2004A版)进行相似度分析。结果：所建立的20批茜草药材及其配方颗粒的TLC法，斑点清晰，分离度良好；建立茜草药材及其配方颗粒的指纹图谱，共有16个共有色谱峰，20批茜草药材及其配方颗粒的指纹图谱相似度 > 0.527，两者化学成分存在一定差异。结论：该研究采用薄层色谱法和HPLC指纹图谱可综合评价茜草药材及其配方颗粒之间的质量差异，从而为茜草及其配方颗粒的质量控制研究提供参考依据。

Abstract: Objective: To establish thin layer chromatography (TLC) and high-performance liquid chromato-graphy (HPLC) fingerprints of Rubia cordifolia L. and its formula granules. Methods: TLC method: The developing agent is petroleum ether-acetone (4:1.3), and it is examined under 365 nm ultra-violet light. HPLC method: The chromatographic column is Diasonsil C18 (4.6 mm × 250 mm), mobile phase is methanol (A)-0.1% phosphoric acid water (B), gradient elution, injection volume 10 μL. The column temperature is 30˚C, and the volume flow rate is 1.0 mL/min. Then, similarity analysis was conducted using the similarity evaluation software for traditional Chinese medicine chromatographic fingerprints (2004A version). Results: The TLC method for the 20 batches of Rubia cordifolia L. and its formula granules established showed clear spots and good separation. HPLC fingerprint of Rubia cordifolia L. and its formula granules was established, 16 common peaks were identified. The similarity of fingerprint spectra of 20 batches of Rubia cordifolia L. and its formula granules is greater than 0.527, indicating certain differences in their chemical composition. Conclusion: This study uses TLC and HPLC fingerprint to comprehensively evaluate the quality differences between Rubia cordifolia L. and its formula granules, thus providing a reference basis for the quality control research of Rubia cordifolia L. and its formula granules.