探究不同浓度七氟醚对七氟醚缺血后处理大鼠的脑保护作用

To Explore Brain Protection Effect of Different Concentration of Sevoflurane on Sevoflurane Ischemic Postconditioning in Rats

摘要: 目的：探究不同浓度七氟醚对七氟醚缺血后处理大鼠的脑保护作用。方法：选取SPF级成年雄性Wistar大鼠，动物被随机分成5组。假手术组、对照组、0.5 MAC组、1.0 MAC组、1.5 MAC组，使用改良的Pulsinelli四血管闭塞法建立脑缺血模型，使用Zea-Longa评分标准进行评分，于再灌注24 h对各组大鼠进行神经功能缺损评分，评分完成后将大鼠(每组8只)以10%水合氯醛腹腔注射麻醉，断头取脑，测量梗死区域大小。结果：神经功能缺陷评分结果与假手术组比较，其他各组24 h缺损功能评分增加(P = 0.05)，与对照组比较，1.0 MAC和1.5 MAC组24 h神经缺损功能评分减轻(P < 0.05)，与0.5 MAC组比较，1.0 MAC和1.5 MAC神经功能缺损评分减轻，1.5 MAC组优于1.0 MAC组(P > 0.05)。脑梗死面积测量结果1.0 MAC和1.5 MAC七氟烷的后处理明显减少了梗死面积，1.0 MAC组的梗死面积为31.2%，1.5 MAC组为27.6%，远小于对照组的梗死面积45.2%，0.5 MAC组的梗死面积为41.5%，与对照组相比梗死面积差距不大。结论：七氟醚对其缺血后处理的大鼠有一定的脑保护保用，且其保护程度与七氟醚浓度存在一定相关性。

Abstract: Objective: To investigate the protective effect of sevoflurane at different concentrations on the brain of rats treated with sevoflurane ischemia. Methods: Adult male Wistar rats with SPF grade were se-lected and randomly divided into 5 groups. In sham operation group, control group, 0.5 MAC group, 1.0 MAC group and 1.5 MAC group, the cerebral ischemia model was established using the modified Pulsinelli four-vessel occlusion method, and the scores were performed using the Zea-Longa scoring standard. The neurological impairment scores were performed for each group at 24 h after reperfu-sion. After the score was completed, the rats (8 in each group) were anesthetized by intraperitoneal injection of 10% chloral hydrate, and the brains were removed by decapitation to measure the in-farct area size. Result: Compared with sham operation group, 24 h defective function score in-creased in other groups (P = 0.05); compared with contrast group, 24 h defective function score de-creased in 1.0 MAC and 1.5 MAC groups (P < 0.05); compared with 0.5 MAC group, 24 h defective function score decreased in 1.0 MAC and 1.5 MAC groups. The neurological impairment scores of 1.0 MAC and 1.5 MAC were reduced, and the 1.5 MAC group was better than the 1.0 MAC group (P > 0.05). The results showed that post-treatment with 1.0 MAC and 1.5 MAC sevoflurane significantly reduced the infarct size. The infarct size of the 1.0 MAC group was 31.2%, and that of the 1.5 MAC group was 27.6%, which was much smaller than that of the control group (45.2%). The infarct size of 0.5 MAC group was 41.5%, which was not much different from that of control group. Conclusion: Sevoflurane can protect the brain of ischemic rats, and the degree of protection is related to the concentration of sevoflurane.